[Studies on the preparation of sample solution for determination of nicotinic acid and nicotinamide in foods by high performance liquid chromatography].

A method for preparation of sample solution for determination of nicotinic acid (NA) and nicotinamide (NAA) in foods by high performance liquid chromatography has been studied. NA and NAA were simultaneously extracted from foods with methanol, then the extracts were cleaned up by use of a Sep-Pak tC18 cartridge. NA and NAA in each sample solution were determined by high performance liquid chromatography. Recoveries of NA and NAA from various foods spiked at levels of 25 and 250 micrograms/g were not less than 70%.