Houf Kurt, De Zutter Lieven, Verbeke Bieke, Van Hoof Jan, Vandamme Peter
Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
J Food Prot. 2003 Mar;66(3):364-9. doi: 10.4315/0362-028x-66.3.364.
In a poultry slaughterhouse, Arcobacter contamination was examined over a period of 1 week to establish possible routes of contamination. Samples were collected from the slaughter equipment and from processing water before the onset of slaughter and from the first broiler flock slaughtered on each sampling day. Characterization of 1,079 isolates by enterobacterial repetitive intergenic consensus-polymerase chain reaction and a random amplified polymorphic DNA assay resulted in the delineation of 159 Arcobacter butzleri and 139 Arcobacter cryaerophilus types. From almost all 140 neck skin samples collected before and after evisceration, A. butzleri and A. cryaerophilus were isolated simultaneously at contamination levels ranging from 10(1) to 10(4) CFU/g. Only six A. butzleri types present in the slaughterhouse environment were also present on the broiler carcasses. None of the A. cryaerophilus genotypes were detected in both the neck skin and the environmental samples. All A. butzleri types isolated from the feather samples were also isolated from broiler neck skin samples. The slaughter equipment was contaminated with arcobacters before the onset of slaughter, but it appeared unlikely that contamination through the slaughter equipment alone explained the high contamination levels on poultry products. Arcobacters were also present in processing water, but types present in water and poultry products were different. Characterization of the Arcobacter isolates did not clarify the routes of transmission, probably because of the extreme heterogeneity among Arcobacter isolates. However, the results obtained in this study brought to light insufficient decontamination at the processing plant involved in the study and confirmed the survival capacity of certain A. butzleri strains.
在一家家禽屠宰场，对1周时间内的弓形杆菌污染情况进行了检测，以确定可能的污染途径。在屠宰开始前，从屠宰设备、加工用水以及每个采样日宰杀的首批肉鸡群中采集样本。通过肠杆菌重复基因间共有序列 - 聚合酶链反应和随机扩增多态性DNA分析对1079株分离菌进行特征分析，结果确定了159种布氏弓形杆菌和139种嗜冷弓形杆菌类型。在几乎所有140份取自主宰前后的颈部皮肤样本中，同时分离出布氏弓形杆菌和嗜冷弓形杆菌，污染水平在10(1)至10(4) CFU/g之间。屠宰场环境中存在的仅六种布氏弓形杆菌类型也出现在肉鸡胴体上。在颈部皮肤和环境样本中均未检测到任何嗜冷弓形杆菌基因型。从羽毛样本中分离出的所有布氏弓形杆菌类型也从肉鸡颈部皮肤样本中分离出来。屠宰设备在屠宰开始前就被弓形杆菌污染，但仅通过屠宰设备造成的污染似乎无法解释禽肉产品上的高污染水平。弓形杆菌也存在于加工用水中，但水中和禽肉产品中存在的类型不同。对弓形杆菌分离菌的特征分析并未明确传播途径，可能是因为弓形杆菌分离菌之间存在极大的异质性。然而，但本研究结果揭示了所涉及的加工厂去污不足的问题，并证实了某些布氏弓形杆菌菌株的存活能力。
