Šilha David, Šilhová-Hrušková Lucie, Vytřasová Jarmila
Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.
Folia Microbiol (Praha). 2015 Nov;60(6):515-21. doi: 10.1007/s12223-015-0395-x. Epub 2015 Apr 26.
This study provides information on the occurrence of Arcobacter in several types of water and food products of animal origin in the Czech Republic. We processed 190 samples using the modified method, and the occurrence of Arcobacter spp. was confirmed in 36.8 % of these. This total incidence consisted of Arcobacter butzleri (27.3 %), Arcobacter cryaerophilus (8.4 %) and Arcobacter skirrowii (1.1 %). We newly described the common presence of Arcobacter spp. in sewage water in the Czech Republic that is released into waterways after processing in water treatment plants (86.7 %). All the acquired isolates were subject to detailed confirmation with subsequent species classification using multiplex PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we used a modification of a method using passive filtration of an enriched sample, which could be suitable for the isolation of Arcobacter, especially in combination with Campylobacter selective agar chromogenic medium. Our studies have shown this agar to be quite suited to the isolation of Arcobacter and that it can be an appropriate instrument for accelerating culture diagnostics.
本研究提供了捷克共和国几种动物源性水和食品中弓形杆菌的发生情况信息。我们采用改良方法处理了190份样本，其中36.8%的样本证实存在弓形杆菌属。这一总发生率包括布氏弓形杆菌（27.3%）、嗜低温弓形杆菌（8.4%）和斯氏弓形杆菌（1.1%）。我们首次描述了捷克共和国污水处理厂处理后排放到水道中的污水中普遍存在弓形杆菌属（86.7%）。所有获得的分离株都经过详细确认，并随后使用多重PCR和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）进行菌种分类。在本研究中，我们对富集样本的被动过滤方法进行了改良，该方法可能适用于弓形杆菌的分离，特别是与弯曲杆菌选择性琼脂显色培养基结合使用时。我们的研究表明，这种琼脂非常适合弓形杆菌的分离，并且它可以成为加速培养诊断的合适工具。
