Gude A, Hillman T J, Helps C R, Allen V M, Corry J E L
University of Bristol, School of Clinical Veterinary Science, Langford, Bristol, UK.
Lett Appl Microbiol. 2005;41(1):82-7. doi: 10.1111/j.1472-765X.2005.01708.x.
To investigate whether Arcobacter spp. colonize the poultry-rearing environment or whether they are contaminants acquired during transportation and/or from the processing plant.
Samples were collected on poultry farms and in the processing plant during slaughter and dressing. Two cultural methods of detection were used. Isolates were identified to species level using a multiplex-polymerase chain reaction (m-PCR) method, either on the initial suspensions, or after enrichment, or on pure cultures of isolates. Of the 62 samples examined from poultry farms, arcobacters were found only outside the rearing sheds (in effluent sludge and stagnant water). Thirty-four samples were examined from the processing plant and 26 were positive for arcobacters. All the isolates were Arcobacter butzleri. Arcobacters were not found in any sample by direct plating nor by m-PCR on the initial suspensions, thus it was concluded that numbers were very low.
Arcobacter spp. were not found in samples from the live birds and their immediate environment, but A. butzleri was found in effluent sludge and stagnant water outside the rearing sheds. However, A. butzleri is common in poultry abattoirs, and it appears that poultry carcasses are contaminated during processing.
Arcobacters are not found inside poultry-rearing sheds, but are contaminants in the processing environment.
调查弓形杆菌属是否定殖于家禽饲养环境，或者它们是否是在运输过程中和/或从加工厂获取的污染物。
在屠宰和脱毛期间，于家禽养殖场和加工厂采集样本。使用了两种培养检测方法。使用多重聚合酶链反应（m-PCR）方法，在初始悬液上、富集后或分离株的纯培养物上，将分离株鉴定到种水平。在家禽养殖场检查的62个样本中，仅在饲养棚外（废水污泥和死水中）发现了弓形杆菌。从加工厂检查了34个样本，其中26个弓形杆菌呈阳性。所有分离株均为布氏弓形杆菌。通过直接平板接种或对初始悬液进行m-PCR，在任何样本中均未发现弓形杆菌，因此得出结论，其数量非常少。
在活禽及其直接环境的样本中未发现弓形杆菌属，但在饲养棚外的废水污泥和死水中发现了布氏弓形杆菌。然而，布氏弓形杆菌在家禽屠宰场很常见，看来家禽胴体在加工过程中受到了污染。
在家禽饲养棚内未发现弓形杆菌，但在加工环境中是污染物。
