Syrigos Konstantinos N, Harrington Kevin J, Karayiannakis Anastasios J, Sekara Eleutheria, Chatziyianni Emmy, Syrigou Ekaterini I, Waxman Jonathan
Laboratory of Molecular Oncology, 3rd Department of Internal Medicine, Sotiria General Hospital, Athens University School of Medicine, Athens, Greece.
Urology. 2003 Mar;61(3):677-80. doi: 10.1016/s0090-4295(02)02289-6.
To investigate the expression of heat shock protein-70 (HSP-70) in transitional cell bladder cancer and correlate it with normal and inflammatory bladder tissue samples.
We used a three-step avidin-biotin method to examine the localization of a rabbit anti-HSP-70 polyclonal antibody in normal (n = 10), inflammatory (n = 10), and malignant (n = 67) bladder tissue samples.
Fifty-eight percent of the bladder cancer samples and only 10% and 20% of the normal and inflammatory bladder tissue samples, respectively, expressed HSP-70. Localization of the antibody correlated with the grade (P <0.001), stage (P <0.001), and survival (P <0.05) of the patients with malignancy.
HSP-70 is frequently overexpressed by bladder cancer cells and could be used as biochemical marker in patients with bladder cancer.
研究热休克蛋白-70（HSP-70）在移行细胞膀胱癌中的表达，并将其与正常膀胱组织和炎性膀胱组织样本进行关联。
我们采用三步抗生物素蛋白-生物素方法，检测兔抗HSP-70多克隆抗体在正常膀胱组织（n = 10）、炎性膀胱组织（n = 10）和恶性膀胱组织（n = 67）样本中的定位。
分别有58%的膀胱癌样本、仅10%的正常膀胱组织样本和20%的炎性膀胱组织样本表达HSP-70。抗体定位与恶性肿瘤患者的分级（P <0.001）、分期（P <0.001）和生存率（P <0.05）相关。
HSP-70在膀胱癌细胞中经常过度表达，可作为膀胱癌患者的生化标志物。
