Marczuk-Krynicka Dorota, Hryniewiecki Tomasz, Piatek Jacek, Paluszak Janusz
Department of Physiology, University School of Medical Sciences Poznań, Poland.
Med Sci Monit. 2003 Mar;9(3):BR131-5.
The aim of our study was to determine the effect of brief starvation on the level of free radicals and other parameters of oxidative status in the liver.
MATERIAL/METHODS: The Wistar male rats used for the experiment were divided into two groups: Group I had free access to water and standard chow, while Group II was subjected to a 36-hour fast. The activity of antioxidant enzymes--catalase (CAT), glutathione peroxidase (GSH-Px) and Cu, Zn-superoxide dysmutase (CuZn-SOD)--was measured in the livers of both control and fasting animals. The hepatic level of free radicals and the concentration of malonyldialdehyde (MDA) were also determined in both groups of animals.
Starvation resulted in decreased activity of CAT and CuZn-SOD, while the GSH-Px activity remained unchanged. Food deprivation also increased the level of free radicals. However, this rise was not paralleled by enhanced hepatic lipid peroxidation, measured as a change in MDA content.
Since brief starvation causes an increase in the level of free radicals in the liver, it appears to effectively impair the tissue defence system, in part through the decreased activity of antioxidant enzymes, CAT and CuZn-SOD.
我们研究的目的是确定短期饥饿对肝脏中自由基水平及氧化状态其他参数的影响。
材料/方法：用于实验的雄性Wistar大鼠分为两组：第一组可自由获取水和标准食物，而第二组禁食36小时。在对照组和禁食组动物的肝脏中测量抗氧化酶——过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和铜锌超氧化物歧化酶（CuZn-SOD）的活性。同时还测定了两组动物肝脏中自由基水平和丙二醛（MDA）浓度。
饥饿导致CAT和CuZn-SOD活性降低，而GSH-Px活性保持不变。食物剥夺也增加了自由基水平。然而，以MDA含量变化衡量的肝脏脂质过氧化作用并未因自由基水平升高而增强。
由于短期饥饿会导致肝脏中自由基水平升高，它似乎会有效损害组织防御系统，部分原因是抗氧化酶CAT和CuZn-SOD的活性降低。
