Sahin Emel, Gümüşlü Saadet
Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey.
Behav Brain Res. 2004 Dec 6;155(2):241-8. doi: 10.1016/j.bbr.2004.04.022.
The aim of this study was to investigate the effects of different stress models on copper, zinc-superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (Se-GSH-Px) activities, and reduced glutathione (GSH), protein carbonyl (PC) and lipid peroxidation marker (conjugated diene (CD) and thiobarbituric acid-reactive substances (TBARS)) levels in brain of rats, and to determine the most effective stress model according to each parameter. Rats were divided into four groups as following: control group (C), immobilization stress group (IS), cold stress group (CS) and immobilization-cold stress group (ICS). All stress models increased brain Cu,Zn-SOD and CAT activities, PC, CD and TBARS levels, plasma corticosterone levels and decreased brain GSH concentrations. Se-GSH-Px activity was increased in CS and ICS groups. When all stress models were taken into consideration, the highest increases in Cu,Zn-SOD and Se-GSH-Px activities were found in CS group. The lowest GSH level was seen in IS group. The highest increases in PC and TBARS levels were found in ICS group. The highest increase of CD concentration was seen in IS and ICS groups. Our results suggest that different stress models have different degrees of influences on enzymatic and non-enzymatic antioxidant defense systems, protein oxidation and lipid peroxidation in the brain.
本研究旨在探讨不同应激模型对大鼠脑内铜、锌超氧化物歧化酶（Cu,Zn-SOD）、过氧化氢酶（CAT）和硒依赖性谷胱甘肽过氧化物酶（Se-GSH-Px）活性，以及还原型谷胱甘肽（GSH）、蛋白质羰基（PC）和脂质过氧化标志物（共轭二烯（CD）和硫代巴比妥酸反应性物质（TBARS））水平的影响，并根据各参数确定最有效的应激模型。大鼠分为以下四组：对照组（C）、束缚应激组（IS）、冷应激组（CS）和束缚-冷应激组（ICS）。所有应激模型均使脑内Cu,Zn-SOD和CAT活性、PC、CD和TBARS水平、血浆皮质酮水平升高，脑内GSH浓度降低。CS组和ICS组Se-GSH-Px活性升高。综合考虑所有应激模型，CS组Cu,Zn-SOD和Se-GSH-Px活性升高幅度最大。IS组GSH水平最低。ICS组PC和TBARS水平升高幅度最大。IS组和ICS组CD浓度升高幅度最大。我们的结果表明，不同应激模型对脑内酶促和非酶促抗氧化防御系统、蛋白质氧化和脂质过氧化有不同程度的影响。
