Parker J H, Mattoon J R
J Bacteriol. 1969 Nov;100(2):647-57. doi: 10.1128/jb.100.2.647-657.1969.
Isolation of a series of mutants, characterized by decreased ability to utilize nonfermentable carbon sources for growth and presence of all cytochromes, is reported. A total of 161 mutants, showing deficient growth on glycerol but able to reduce 2,3,5-triphenyltetrazolium chloride, were isolated, purified, and characterized by ability to grow on various carbon sources. Mutants showing decreased growth were examined by low-temperature spectroscopy, and the 35 strains shown to possess all cytochromes were retained for further studies. These strains were characterized by growth on various nonfermentable carbon sources, relative yield on glucose medium, and respiration (Q(O2)) of glucose and ethyl alcohol. Genetic studies revealed that at least 19 of the 35 mutants are the result of mutation in single nuclear genes. Furthermore, at least 11 complementation groups are represented among these 19 mutants. Mutants within two complementation groups were shown to be very similar in various properties. These studies demonstrate that a large number of nuclear genes control oxidative energy metabolism and that the characteristics of mutants of the general class are extremely diverse.
报道了一系列突变体的分离情况，其特征为利用非发酵性碳源进行生长的能力下降且存在所有细胞色素。总共分离出161个突变体，它们在甘油上生长不足但能够还原氯化三苯基四氮唑，经过纯化，并通过在各种碳源上生长的能力进行表征。对生长减少的突变体进行低温光谱检查，保留了显示具有所有细胞色素的35个菌株用于进一步研究。这些菌株通过在各种非发酵性碳源上的生长、在葡萄糖培养基上的相对产量以及葡萄糖和乙醇的呼吸作用（Q(O2)）进行表征。遗传研究表明，35个突变体中至少有19个是单核基因突变的结果。此外，这19个突变体中至少代表了11个互补群。两个互补群内的突变体在各种特性上非常相似。这些研究表明，大量的核基因控制氧化能量代谢，并且这类一般突变体的特征极其多样。
