脂质-F1核组蛋白相互作用

Lipid-F1 nucleohistone interactions.

作者信息

Manzoli F A, Muchmore J H, Capitani S, Bonora B, Bartoli S

出版信息

Mol Cell Biochem. 1976 Feb 25;10(3):153-60. doi: 10.1007/BF01731686.

Abstract

High concentrations of phospholipids determine destabilization of F1 histone-DNA complex at the weight ratios, histone:DNA, 0.8:1 and 1:1, but low concentrations cause only negligible destabilization. Cholesterol at high weight ratios has little effect on nucleohistone stability. Only linolenic acid of the fatty acids used reproduces similar changes in the thermal stability of F1 histone-DNA complex as phospholipids. The type of interaction of phospholipids with the F1 histone-DNA complex is analyzed, and the involvement of phospholipids in DNA replication in vivo is discussed.

摘要

高浓度磷脂会在组蛋白与DNA重量比为0.8:1和1:1时导致F1组蛋白-DNA复合物不稳定，但低浓度时只会引起可忽略不计的不稳定。高重量比的胆固醇对核组蛋白稳定性影响很小。在所使用的脂肪酸中，只有亚麻酸能像磷脂一样使F1组蛋白-DNA复合物的热稳定性产生类似变化。分析了磷脂与F1组蛋白-DNA复合物的相互作用类型，并讨论了磷脂在体内DNA复制中的作用。