DNA链的柔韧性与染色质核小体的结构

DNA chain flexibility and the structure of chromatin nu-bodies.

作者信息

Harrington R E

出版信息

Nucleic Acids Res. 1977 Oct;4(10):3519-35. doi: 10.1093/nar/4.10.3519.

DOI:10.1093/nar/4.10.3519

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342669/

Abstract

The persistence length of high-molecular-weight, monodisperse-bihelical DNA has been evaluated from low-shear flow birefingence and viscosity data at several temperatures in 2.0 M Nacl neutral pH buffer. At these solvent conditions, both the DNA and histone components of chromatin nu-bodies have structural features similar to those in the intact nucleohistone complex at low ionic strength. The theory of Landau and Lifshitz is used to relate the experimental result to the thermodynamic functions for bending 140 nucleotide pairs of DNA into a plausible model structure: per nu-body, delta Gb=43.8 +/- 5.3 kcal/mole, delta Hb= 45.7 +/- 3.7 kcal/mole, and delta Sb = 6.2 +/- 12.4 entropy units. This bending free energy is comparable to or less than that estimated to be required for a kinked DNA configuration and appears to be well within the range of estimated electrostatic free energies available from DNA-histone interactions in a nu-body assembly.

摘要

在2.0M氯化钠中性pH缓冲液中，于几个温度下，通过低剪切流双折射和粘度数据评估了高分子量、单分散双螺旋DNA的持续长度。在这些溶剂条件下，染色质核小体的DNA和组蛋白成分都具有与低离子强度下完整核组蛋白复合物相似的结构特征。利用朗道和利夫希茨理论将实验结果与将140个核苷酸对的DNA弯曲成合理模型结构的热力学函数相关联：每个核小体，ΔGb = 43.8±5.3千卡/摩尔，ΔHb = 45.7±3.7千卡/摩尔，以及ΔSb = 6.2±12.4熵单位。这种弯曲自由能与估计的扭结DNA构型所需的自由能相当或更低，并且似乎完全在核小体组装中DNA-组蛋白相互作用可获得的估计静电自由能范围内。

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J Mol Biol. 1961 Oct;3:547-65. doi: 10.1016/s0022-2836(61)80021-1.

2

Degradation of deoxyribonucleic acid under hydrodynamic shearing forces.在流体动力学剪切力作用下脱氧核糖核酸的降解

J Mol Biol. 1961 Oct;3:674-83. doi: 10.1016/s0022-2836(61)80030-2.

3

Structure of T-2 bacteriophage.T-2噬菌体的结构。

Nature. 1962 Aug 4;195:494-5. doi: 10.1038/195494a0.

4

The molecular weights of T2 bacteriophage DNA and its first and second breakage products.T2噬菌体DNA及其一级和二级断裂产物的分子量。

Proc Natl Acad Sci U S A. 1961 Aug;47(8):1113-22. doi: 10.1073/pnas.47.8.1113.

5

The structural unity of the DNA of T2 bacteriophage.T2噬菌体DNA的结构统一性

Proc Natl Acad Sci U S A. 1961 Aug;47(8):1123-9. doi: 10.1073/pnas.47.8.1123.

6

A model for particulate structure in chromatin.染色质中颗粒结构的模型。

Nucleic Acids Res. 1974 Nov;1(11):1579-86. doi: 10.1093/nar/1.11.1579.

7

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8

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