Fischer J, Romhányi G
Morphol Igazsagugyi Orv Sz. 1976 Apr;16(2):137-44.
Reaction with aldehyd-bisulfit-toluidinblue (ABT)--as a selective topo-optical reaction for vicinal OH-and amino-OH groups, appears to be suitable for demonstration of bacteria in tissues. Cell membranes and capsules of bacteria and fungi contain large quantity of carbo-hydrates, therefore they show strong basophilic staining after ABT reaction. Double refringement brought about by orientated dye-binding seems to be the sign of the linear arrangement of polysaccharides composing cell membranes and capsules of bacteria. Practical importance of the reaction is shown on examples. Diagnostic advantages of it are also discussed.
与醛-亚硫酸氢盐-甲苯胺蓝（ABT）的反应——作为一种针对邻位羟基和氨基羟基的选择性拓扑光学反应，似乎适用于显示组织中的细菌。细菌和真菌的细胞膜及荚膜含有大量碳水化合物，因此在ABT反应后呈现出强烈的嗜碱性染色。由定向染料结合引起的双折射似乎是构成细菌细胞膜和荚膜的多糖线性排列的标志。通过实例展示了该反应的实际重要性。还讨论了其诊断优势。
