Romháyi G, Németh A, György D, Deák G
Morphol Igazsagugyi Orv Sz. 1976 Apr;16(2):126-36.
Topo-optical reaction with aldehyd-bisulfit-toluidinblue (ABT) suitable for the demonstration of lineal arrangement of vicinal OH-radicals is described. On the base of double refringement brought about by the orientated dye-binding, this reaction is suitable for the study of molecular arrangement of carbohydrate components of biological structures (glycoproteins, glycolipides and polysaccharides). Double refringement brought about by the reaction ABT optically is negative to the length of polysaccharide-chains. Practical use of the method is demonstrated on several biological structures. Findings show, that reaction ABT opens new possibilities for the study of ultrastructure of carbohydrate components of various tissue elements.
描述了一种与醛-亚硫酸氢盐-甲苯胺蓝（ABT）的拓扑光学反应，该反应适用于显示邻位羟基自由基的线性排列。基于定向染料结合引起的双折射，此反应适用于研究生物结构（糖蛋白、糖脂和多糖）中碳水化合物成分的分子排列。ABT反应引起的双折射在光学上与多糖链的长度呈负相关。在几种生物结构上展示了该方法的实际应用。研究结果表明，ABT反应为研究各种组织成分中碳水化合物成分的超微结构开辟了新的可能性。
