[Topo-optical demonstration of lineal arrangement of vicial OH radicals by means of aldehyde bisulfite toluidine blue (ABT) reaction].

Topo-optical reaction with aldehyd-bisulfit-toluidinblue (ABT) suitable for the demonstration of lineal arrangement of vicinal OH-radicals is described. On the base of double refringement brought about by the orientated dye-binding, this reaction is suitable for the study of molecular arrangement of carbohydrate components of biological structures (glycoproteins, glycolipides and polysaccharides). Double refringement brought about by the reaction ABT optically is negative to the length of polysaccharide-chains. Practical use of the method is demonstrated on several biological structures. Findings show, that reaction ABT opens new possibilities for the study of ultrastructure of carbohydrate components of various tissue elements.