Saeedi Baharak, Hällgren Anita, Jonasson Jon, Nilsson Lennart E, Hanberger Håkan, Isaksson Barbro
Division of Clinical Microbiology, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
APMIS. 2002 Dec;110(12):869-74. doi: 10.1034/j.1600-0463.2002.1101205.x.
Controlling the spread of vancomycin-resistant enterococci (VRE) is an important task in hospital epidemiology. Pulsed-field gel electrophoresis (PFGE) has become the golden standard for molecular epidemiological characterisation of enterococcal isolates. For separation of DNA fragments by PFGE, different electrophoresis conditions have been recommended, but none of these protocols allows a satisfactory separation of both small and large DNA fragments of enterococci simultaneously. In this study we have speeded up the preparation of chromosomal DNA and defined new electrophoresis conditions that enhance separation of small and large DNA fragments for subtyping of enterococci with a 24 h PFGE.
控制耐万古霉素肠球菌（VRE）的传播是医院流行病学中的一项重要任务。脉冲场凝胶电泳（PFGE）已成为肠球菌分离株分子流行病学特征分析的金标准。对于通过PFGE分离DNA片段，已推荐了不同的电泳条件，但这些方案均无法同时令人满意地分离肠球菌的小DNA片段和大DNA片段。在本研究中，我们加快了染色体DNA的制备，并确定了新的电泳条件，可通过24小时PFGE增强肠球菌小DNA片段和大DNA片段的分离，用于亚型分析。
