Cambi Alessandra, Gijzen Karlijn, de Vries l Jolanda M, Torensma Ruurd, Joosten Ben, Adema Gosse J, Netea Mihai G, Kullberg Bart-Jan, Romani Luigina, Figdor Carl G
Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, Nijmegen, The Netherlands.
Eur J Immunol. 2003 Feb;33(2):532-8. doi: 10.1002/immu.200310029.
Dendritic cells (DC) that express the type II C-type lectin DC-SIGN (CD209) are located in the submucosa of tissues, where they mediate HIV-1 entry. Interestingly, the pathogen Candida albicans, the major cause of hospital-acquired fungal infections, penetrates at similar submucosal sites. Here we demonstrate that DC-SIGN is able to bind C. albicans both in DC-SIGN-transfected cell lines and in human monocyte-derived DC. The binding was shown to be time- as well as concentration-dependent, and live as well as heat-inactivated C. albicans were bound to the same extent. Moreover, in immature DC, DC-SIGN was able to internalize C. albicans in specific DC-SIGN-enriched vesicles, distinct from those containing the mannose receptor, the other known C. albicans receptor expressed by DC. Together, these results demonstrate that DC-SIGN is an exquisite pathogen-uptake receptor that captures not only viruses but also fungi.
表达II型C型凝集素DC-SIGN（CD209）的树突状细胞（DC）位于组织的黏膜下层，在那里介导HIV-1的进入。有趣的是，医院获得性真菌感染的主要病原体白色念珠菌也在类似的黏膜下部位侵入。在此我们证明，DC-SIGN在转染DC-SIGN的细胞系以及人单核细胞衍生的DC中均能够结合白色念珠菌。结果表明，这种结合具有时间和浓度依赖性，活的以及热灭活的白色念珠菌的结合程度相同。此外，在未成熟DC中，DC-SIGN能够将白色念珠菌内化到特定的富含DC-SIGN的囊泡中，这些囊泡与含有甘露糖受体（DC表达的另一种已知的白色念珠菌受体）的囊泡不同。总之，这些结果表明，DC-SIGN是一种精巧的病原体摄取受体，不仅能捕获病毒，还能捕获真菌。
