Naik Shalin, Vremec David, Wu Li, O'Keeffe Meredith, Shortman Ken
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Blood. 2003 Jul 15;102(2):601-4. doi: 10.1182/blood-2002-10-3186. Epub 2003 Mar 20.
Although previous studies had indicated that the CD8alpha- and CD8alpha+ subtypes of murine dendritic cells (DCs) differ in immediate origin, a recent study found that intravenous transfer of CD8alpha- DCs led to CD8alpha+ DCs in the spleen several days later, suggesting a direct precursor-product relationship. We have repeated these experiments with a balance sheet approach. We find that though a few CD8alpha+ DCs can be generated in such experiments, this is a rare event and could be the result of a contaminant precursor. Most of the immediate precursors of CD8alpha+ DCs are cells that lack the phenotype of a recognizable DC. CD8alpha- DCs and CD8alpha+ DCs are not precursor-product related, though these sublineages may be connected further upstream.
尽管先前的研究表明，小鼠树突状细胞（DCs）的CD8α - 和CD8α + 亚型在直接起源上有所不同，但最近的一项研究发现，静脉注射CD8α - DCs几天后会在脾脏中产生CD8α + DCs，这表明存在直接的前体 - 产物关系。我们采用平衡表方法重复了这些实验。我们发现，虽然在这类实验中可以产生少数CD8α + DCs，但这是一个罕见事件，可能是污染前体的结果。CD8α + DCs的大多数直接前体是缺乏可识别DC表型的细胞。CD8α - DCs和CD8α + DCs并非前体 - 产物关系，尽管这些亚谱系可能在更上游存在联系。
