Min Kwangseon, Hong Seung Mo, Kim Kyu Rae, Ro Jae Y, Park Min Jung, Kim Joo Sung, Kim Jung Mogg, Jung Hyun Chae, Yu Eunsil
Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Pathol Res Pract. 2003;199(1):1-8. doi: 10.1078/0344-0338-00345.
Intramucosal Helicobacter pylori (H. pylori) has been described in biopsy tissues and culture systems. However, the association of intramucosal H. pylori with histopathologic features has not been evaluated. The purpose of this study is to investigate the relationship between intramucosal H. pylori and inflammatory reactions in H. pylori infection. In 113 randomly selected human gastric biopsies and 20 murine stomachs, which were inoculated with SSI every day for a week, immunohistochemical analysis for intramucosal H. pylori was done and correlated with histologic parameters. Electron microscopic examination was done on murine stomachs. H. pylori infection was present in 104 gastric biopsies and 17 murine stomachs. Intraepithelial immunopositivity for H. pylori was detected in 27 of 104 (26%) biopsies and in 11 of 17 (65%) murine stomachs. Lamina proprial immunopositivity for H. pylori was present in 51 of 104 (48%) biopsies. Neutrophil-associated immunopositivity for H. pylori was observed in 22 of 90 (24%) biopsies with H. pylori chronic active gastritis. Lamina proprial and neutrophil-associated immunopositivity for H. pylori correlated significantly with the density of H. pylori and the grade of acute inflammatory reaction in H. pylori gastritis. Intramucosal location of H. pylori itself or its antigen is closely associated with acute inflammatory reactions and may play an important role in establishing a persistent infection in chronic H. pylori gastritis. Furthermore, lamina proprial and/or neutrophil-associated H. pylori appears to be more important than intraepithelial H. pylori in acute inflammatory reactions of H. pylori gastritis.
黏膜内幽门螺杆菌（H. pylori）已在活检组织和培养系统中被描述。然而，黏膜内幽门螺杆菌与组织病理学特征之间的关联尚未得到评估。本研究的目的是探讨黏膜内幽门螺杆菌与幽门螺杆菌感染中炎症反应之间的关系。在113例随机选取的人胃活检组织和20只小鼠胃中，每天接种标准菌株（SSI），持续一周，对黏膜内幽门螺杆菌进行免疫组织化学分析，并与组织学参数相关联。对小鼠胃进行电子显微镜检查。104例胃活检组织和17只小鼠胃中存在幽门螺杆菌感染。在104例活检组织中的27例（26%）和17只小鼠胃中的11例（65%）检测到幽门螺杆菌的上皮内免疫阳性。在104例活检组织中的51例（48%）检测到幽门螺杆菌的固有层免疫阳性。在90例幽门螺杆菌慢性活动性胃炎活检组织中的22例（24%）观察到与中性粒细胞相关的幽门螺杆菌免疫阳性。幽门螺杆菌的固有层和与中性粒细胞相关的免疫阳性与幽门螺杆菌胃炎中幽门螺杆菌的密度和急性炎症反应程度显著相关。幽门螺杆菌本身或其抗原在黏膜内的定位与急性炎症反应密切相关，可能在慢性幽门螺杆菌胃炎持续感染的建立中起重要作用。此外，在幽门螺杆菌胃炎的急性炎症反应中，固有层和/或与中性粒细胞相关的幽门螺杆菌似乎比上皮内幽门螺杆菌更重要。
