血浆中吲哚 - 3 - 甲醇及其酸缩合产物的液相色谱同时测定法。
Liquid chromatographic assay for the simultaneous determination of indole-3-carbinol and its acid condensation products in plasma.
作者信息
Anderton Mark J, Jukes Rebekah, Lamb John H, Manson Margaret M, Gescher Andreas, Steward William P, Williams Marion L
机构信息
Department of Oncology, University of Leicester, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, UK.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Apr 25;787(2):281-91. doi: 10.1016/s1570-0232(02)00923-6.
A high-performance liquid chromatographic method was developed for the simultaneous determination of indole-3-carbinol (I3C), 3,3'-diindolylmethane (DIM), [2-(indol-3-ylmethyl)-indol-3-yl]indol-3-ylmethane (LTr(1)), and indolo[3,2b]carbazole (ICZ). Compounds were extracted from mouse plasma using tert.-butyl methyl ether, incorporating 4-methoxy-indole as internal standard. Chromatographic separation utilized a Waters Symmetry RP18 in tandem with a Thermoquest BDS C(18) column, an acetonitrile-water gradient and UV (280 nm) in series with fluorescence (ex. 335 nm; em. 415 nm) detection. Calibration curves were linear (r(2)>0.99) between 50 and 15,000 ng/ml for I3C; 150 and 15,000 ng/ml for LTr(1); and 0.15 and 37.5 ng/ml for ICZ and the method was reproducible and precise (within-day and between-day coefficients of variation below 9.7 and 13%, respectively). The method described is suitable for comprehensive pharmacokinetic studies with indole-3-carbinol.
建立了一种高效液相色谱法，用于同时测定吲哚 - 3 - 甲醇（I3C）、3,3'-二吲哚基甲烷（DIM）、[2 -（吲哚 - 3 - 基甲基）-吲哚 - 3 - 基]吲哚 - 3 - 基甲烷（LTr(1)）和吲哚并[3,2 - b]咔唑（ICZ）。使用叔丁基甲醚从小鼠血浆中提取化合物，并加入4 - 甲氧基吲哚作为内标。色谱分离采用沃特世Symmetry RP18与热电赛默飞世尔BDS C(18)柱串联，乙腈 - 水梯度洗脱，并串联紫外（280 nm）和荧光（激发波长335 nm；发射波长415 nm）检测。I3C在50至15,000 ng/ml之间、LTr(1)在150至15,000 ng/ml之间以及ICZ在0.15至37.5 ng/ml之间的校准曲线呈线性（r(2)>0.99），该方法具有可重复性和精密度（日内和日间变异系数分别低于9.7%和13%）。所描述的方法适用于吲哚 - 3 - 甲醇的全面药代动力学研究。
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