Järbe T U, Johansson J O
Pharmacol Biochem Behav. 1976 Feb;4(2):151-7. doi: 10.1016/0091-3057(76)90007-1.
Groups of rats were trained in a T-shaped maze to discriminate the effects produced by IP injections of ditran (1.60 mg/kg), either when given singly, or when combined with the acetylcholinesterase inhibitors neostigmine (0.25 mg/kg) or physostigmine (0.50 and 1.00 mg/kg), from the nondrug condition (saline). The results from this state-dependency (StD) model indicated that acquisition of the drug discrimination was similar for the 4 groups of rats. After drug discrimination was established the rats were tested with various drug combinations. Physostigmine (0.50 and 1.00 mg/kg) challenge reversed drug discrimination among rats trained with ditran solely or the ditran plus neostigmine combination. There was no antagonism among the ditran plus physostigmine trained rats. Involvement of the C.N.S. is implicated since tests with neostigmine did not upset ditran discrimination. In addition, survival rate of physostigmine treated mice is increased with ditran. In conclusion, this study indicates the usefulness of employing both training and transfer test procedures when evaluating antagonism in this StD model.
将大鼠分成几组，在T型迷宫中进行训练，以区分腹腔注射地卓西平马来酸盐（1.60毫克/千克）单独给药时，或与乙酰胆碱酯酶抑制剂新斯的明（0.25毫克/千克）或毒扁豆碱（0.50和1.00毫克/千克）联合给药时，与非药物状态（生理盐水）所产生的效果。这种状态依赖性（StD）模型的结果表明，4组大鼠对药物辨别力的习得情况相似。在建立药物辨别力后，用各种药物组合对大鼠进行测试。毒扁豆碱（0.50和1.00毫克/千克）激发试验逆转了仅用地卓西平马来酸盐训练的大鼠或地卓西平马来酸盐加新斯的明组合训练的大鼠之间的药物辨别。在地卓西平马来酸盐加毒扁豆碱训练的大鼠之间没有拮抗作用。由于新斯的明测试并未扰乱地卓西平马来酸盐的辨别，因此表明中枢神经系统参与其中。此外，毒扁豆碱处理的小鼠与地卓西平马来酸盐一起时存活率会提高。总之，本研究表明在这个状态依赖性模型中评估拮抗作用时，采用训练和转移测试程序的有用性。
