Gedymin L E, Erokhin V V, Lepekha L N, Nikolaeva G M, Kaminskaia G O, Kuznetsov V P, Beliaev D L, Babaiants A A
Probl Tuberk. 2003(1):45-52.
An experiment was conducted on 60 CBA mice intravenously inoculated with cultured Mycobacteria tuberculosis (MBT), Erdmann strain, in a dose of 0.025 mg. The specific features of tissue, cellular, and biochemical reactions were studied in the lung, liver, and spleen when leukinferon (LF) was included into tuberculosis treatment regimen. LF was shown to have a positive impact on the development of reparative reactions during tuberculous inflammation by reducing the time of abacillation and recovering the structure of diseased organs. By month 3 of follow-up, MBT were not detected in mice receiving antibacterial agents (ABA) and LF, while typical and changed forms of LF were identified in the cytoplasm of alveolar macrophages in mice treated with ABA alone. A specific feature of an inflammatory reaction as a significant proliferation of lymphocytes and macrophages with their ample infiltration of target organs was noted in animals receiving LF. This was followed by the activated production of alpha- and gamma-interferons and by the mobilization of an enzymatic link of anti-oxidant defense under chronic oxidative stress, which led to a reduction of resolution of inflammatory areas and to an increase in survival of animals which had not been given ABA, but treated with LF alone.
对60只静脉接种0.025毫克培养的结核分枝杆菌（MBT） Erdmann菌株的CBA小鼠进行了一项实验。当将白细胞干扰素（LF）纳入结核病治疗方案时，研究了肺、肝和脾组织、细胞及生化反应的特定特征。结果表明，LF通过缩短脱痂时间和恢复患病器官结构，对结核性炎症期间修复反应的发展具有积极影响。到随访第3个月时，在接受抗菌剂（ABA）和LF的小鼠中未检测到MBT，而在仅用ABA治疗的小鼠肺泡巨噬细胞胞质中鉴定出典型和变化形式的LF。在接受LF的动物中，炎症反应的一个特定特征是淋巴细胞和巨噬细胞显著增殖，并大量浸润靶器官。随后是α干扰素和γ干扰素的激活产生，以及在慢性氧化应激下动员抗氧化防御的酶促环节，这导致炎症区域消退减少，未给予ABA但仅用LF治疗的动物存活率增加。
