Ozer F, Karakaya S, Unlü N, Erganiş O, Kav K, Imazato S
Department of Operative Dentistry, Faculty of Dentistry, University of Selcuk, Konya, Turkey.
J Dent. 2003 Feb;31(2):111-6. doi: 10.1016/s0300-5712(03)00006-x.
This study compared the antibacterial activities of two dentin bonding systems (ABF, Kuraray and Reactmer Bond, Shofu) by a conventional agar well technique and a newly designed in vitro test using tooth model.
In the agar well technique, the test materials were filled in the wells of Muller Hinton agar plates inoculated with Streptococcus mutans NCTC10449, and the diameters of inhibition zones produced around the materials were measured after 24h of incubation. For the tooth model test, three cavities (diameter 1mm, depth 2mm) were prepared in the flat occlusal dentin of human extracted molar. After sterilization, the teeth were left in broth culture of 1.56 x 10(8)CFU/ml of S. mutans at 37 degrees C for 72h for allowing bacteria to invade the cavity. The dentin bonding systems were applied separately to each of the two infected cavities, and the third cavity was left unapplied for control. After sealing the occlusal surfaces, the teeth were kept in physiologic saline solution at 37 degrees C for 72h. The standardized amounts of dentin chips (120+/-5mg) were obtained from the cavity walls and the number of bacteria recovered was determined. The results were analyzed by One Way ANOVA, Kruskal Wallis and Mann-Whitney's U tests.
The primer of ABF and Reactmer Bond produced inhibition zones with similar sizes (p>0.05), but the bonding resin of ABF did not produce any inhibition. When tested by the model cavity method, the application of ABF resulted in significantly less bacterial recovery than Reactmer Bond (p<0.05), demonstrating substantial antibacterial effects.
The tooth model method used in this study was effective for evaluating the substantial antibacterial effects of dentin bonding agents, and the experimental dentin bonding system ABF was demonstrated to be able to inactivate the bacteria in the cavity effectively in comparison with little antibacterial activity shown by Reactmer Bond.
本研究采用传统的琼脂孔技术和一种新设计的使用牙齿模型的体外试验，比较两种牙本质粘结系统（ABF，可乐丽公司；Reactmer Bond，松风公司）的抗菌活性。
在琼脂孔技术中，将测试材料填充到接种了变形链球菌NCTC10449的Muller Hinton琼脂平板的孔中，培养24小时后测量材料周围产生的抑菌圈直径。对于牙齿模型试验，在人离体磨牙的平坦咬合牙本质上制备三个洞（直径1mm，深度2mm）。灭菌后，将牙齿置于含有1.56×10⁸CFU/ml变形链球菌的肉汤培养物中，在37℃下培养72小时，以使细菌侵入洞中。将牙本质粘结系统分别应用于两个感染洞中的每一个，第三个洞不应用作为对照。封闭咬合面后，将牙齿置于37℃的生理盐水中72小时。从洞壁获取标准化量的牙本质碎屑（120±5mg），并测定回收的细菌数量。结果采用单因素方差分析、Kruskal Wallis检验和Mann-Whitney U检验进行分析。
ABF和Reactmer Bond的底漆产生的抑菌圈大小相似（p>0.05），但ABF的粘结树脂未产生任何抑菌作用。当通过模型洞方法测试时，应用ABF导致回收的细菌数量明显少于Reactmer Bond（p<0.05），表明具有显著的抗菌效果。
本研究中使用的牙齿模型方法对于评估牙本质粘结剂的实际抗菌效果是有效的，并且与Reactmer Bond显示的几乎没有抗菌活性相比，实验性牙本质粘结系统ABF被证明能够有效地使洞中细菌失活。
