Lukomska-Szymanska Monika, Konieczka Magdalena, Zarzycka Beata, Lapinska Barbara, Grzegorczyk Janina, Sokolowski Jerzy
Department of General Dentistry, Medical University of Lodz, Lodz 92-213, Poland.
Department of Microbiology and Laboratory Medical Immunology, Medical University of Lodz, Lodz 92-213, Poland.
Materials (Basel). 2017 Apr 29;10(5):481. doi: 10.3390/ma10050481.
Literature presents inconsistent results on the antibacterial activity of dentine bonding systems (DBS). Antibacterial activity of adhesive systems depends on several factors, including composition and acidity. Flow cytometry is a novel detection method to measure multiple characteristics of a single cell: total cell number, structural (size, shape), and functional parameters (viability, cell cycle). The LIVE/DEAD® BacLightTM bacterial viability assay was used to evaluate an antibacterial activity of DBS by assessing physical membrane disruption of bacteria mediated by DBS. Ten commercial DBSs: four total-etching (TE), four self-etching (SE) and two selective enamel etching (SEE) were tested. Both total-etching DBS ExciTE F and OptiBond Solo Plus showed comparatively low antibacterial activity against E. faecalis. The lowest activity of all tested TE systems showed Te-Econom Bond. Among SE DBS, G-ænial Bond (92.24% dead cells) followed by Clearfil S3 Bond Plus (88.02%) and Panavia F 2.0 ED Primer II (86.67%) showed the highest antibacterial activity against E. faecalis, which was comparable to isopropranol (positive control). In the present study, self-etching DBS exhibited higher antimicrobial activity than tested total-etching adhesives against E. faecalis.
文献中关于牙本质粘结系统（DBS）的抗菌活性呈现出不一致的结果。粘结系统的抗菌活性取决于多个因素，包括成分和酸度。流式细胞术是一种用于测量单个细胞多种特性的新型检测方法：细胞总数、结构（大小、形状）和功能参数（活力、细胞周期）。采用LIVE/DEAD® BacLightTM细菌活力检测法，通过评估DBS介导的细菌细胞膜物理破坏情况来评价DBS的抗菌活性。测试了十种市售DBS：四种全酸蚀（TE）、四种自酸蚀（SE）和两种选择性釉质酸蚀（SEE）。全酸蚀DBS ExciTE F和OptiBond Solo Plus对粪肠球菌均表现出相对较低的抗菌活性。所有测试的TE系统中，Te-Econom Bond的活性最低。在SE DBS中，G-ænial Bond（92.24%死细胞），其次是Clearfil S3 Bond Plus（88.02%）和Panavia F 2.0 ED Primer II（86.67%）对粪肠球菌表现出最高的抗菌活性，这与异丙醇（阳性对照）相当。在本研究中，自酸蚀DBS对粪肠球菌的抗菌活性高于测试的全酸蚀粘结剂。
