Rainey Fred A, Silva Joana, Nobre M Fernanda, Silva Manuel T, da Costa Milton S
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):35-41. doi: 10.1099/ijs.0.02308-0.
Two strains of a novel aerobic, bacteriochlorophyll a-containing species of the alpha-4 subclass of the Proteobacteria were isolated from the hot spring at Alcafache in central Portugal. 16S rRNA gene sequence-based phylogenetic analyses showed the two novel isolates to be phylogenetically related to members of the genera Erythrobacter, Erythromicrobium and Porphyrobacter. The strains produce reddish-orange-pigmented colonies, have an optimum growth temperature of about 50 degrees C and could be distinguished from the species Porphyrobacter tepidarius, which also has a high growth temperature, primarily on the basis of the fatty acid composition. The novel species does not grow anaerobically in the presence or absence of a light source. The strains of the novel species utilize several single carbon sources for growth, most of which are also used by P. tepidarius. The species status of strains ALC-2T and ALC-3 was confirmed by low reassociation values of the DNA with species of the genera Erythrobacter, Erythromicrobium and Porphyrobacter. Phenotypic characteristics and 16S rRNA gene sequence analyses also show that strains ALC-2T (=DSM 12079T =ATCC BAA-386T) and ALC-3 (=DSM 12080) represent a novel species, for which the name Porphyrobacter cryptus sp. nov. is proposed.
从葡萄牙中部阿尔卡法什的温泉中分离出两株新型需氧菌，属于变形菌门α-4亚类，含有细菌叶绿素a。基于16S rRNA基因序列的系统发育分析表明，这两株新分离菌株在系统发育上与红杆菌属、红微菌属和卟啉杆菌属的成员相关。这些菌株产生红橙色色素菌落，最适生长温度约为50℃，并且主要基于脂肪酸组成，可与同样具有较高生长温度的嗜温卟啉杆菌区分开来。该新物种在有或无光源的情况下均不能厌氧生长。新物种的菌株利用多种单一碳源进行生长，其中大多数也是嗜温卟啉杆菌所利用的。菌株ALC-2T和ALC-3与红杆菌属、红微菌属和卟啉杆菌属物种的DNA低重结合值证实了它们的物种地位。表型特征和16S rRNA基因序列分析还表明，菌株ALC-2T（=DSM 12079T =ATCC BAA-386T）和ALC-3（=DSM 12080）代表一个新物种，为此提出新名称嗜温卟啉杆菌（Porphyrobacter cryptus sp. nov.）。
