Hanada S, Kawase Y, Hiraishi A, Takaichi S, Matsuura K, Shimada K, Nagashima K V
Department of Biology, Tokyo Metropolitan University, Hachioji, Japan.
Int J Syst Bacteriol. 1997 Apr;47(2):408-13. doi: 10.1099/00207713-47-2-408.
A new thermophilic bacterium, strain OT3T (T = type strain), was isolated from a brackish hot spring. Strain OT3T is an obligate aerobe that synthesizes bacteriochlorophyll a and has a photosynthetic apparatus. This isolate is a thermophilic bacterium with an optimal growth temperature of 40 to 48 degrees C. The cells are nonmotile, ovoid to short rods. An analysis of 16S rRNA sequences revealed that the new strain forms a coherent cluster with members of the alpha-4 group of the alpha subclass of the Proteobacteria, which contains the genera Erythrobacter, Erythromicrobium, and Porphyrobacter. The closest relative is Porphyrobacter neustonensis, with a 16S rRNA sequence similarity of 96.8%. The in vivo absorption spectrum has maxima at 460, 494, 596, 800, and 870 nm. The main carotenoids are OH-beta-carotene sulfate derivatives, nostoxanthin, and bacteriorubixanthinal. Growth occurs with glucose, acetate, glutamate, butyrate, Casamino Acids, and yeast extract as sole energy sources. The pigment composition and nutritional profile of the new isolate are similar to the pigment composition and nutritional profile of P. neustonensis. Although there are marked differences in cell morphology between the new isolate and the budding bacterium P. neustonensis, the results of phenotypic and genetic comparisons suggest that the new isolate is closely related to P. neustonensis. Consequently, we assign the new isolate to the genus Porphyrobacter and propose the name Porphyrobacter tepidarius sp. nov. for it; the type strain of P. tepidarius is strain OT3 (= DSM 10595).
从微咸温泉中分离出一种新的嗜热细菌，菌株OT3T（T = 模式菌株）。菌株OT3T是一种专性需氧菌，能合成细菌叶绿素a并具有光合装置。该分离株是一种嗜热细菌，最佳生长温度为40至48摄氏度。细胞无运动性，呈卵形至短杆状。16S rRNA序列分析表明，新菌株与变形菌门α亚类的α-4组成员形成一个连贯的聚类，该组包含红杆菌属、红微菌属和卟啉杆菌属。最接近的亲缘种是海洋卟啉杆菌，16S rRNA序列相似度为96.8%。体内吸收光谱在460、494、596、800和870 nm处有最大值。主要类胡萝卜素是羟基-β-胡萝卜素硫酸酯衍生物、藻胆黄质和细菌红素黄醛。以葡萄糖、乙酸盐、谷氨酸盐、丁酸盐、酪蛋白氨基酸和酵母提取物作为唯一能源时可生长。新分离株的色素组成和营养特征与海洋卟啉杆菌的色素组成和营养特征相似。尽管新分离株与出芽细菌海洋卟啉杆菌在细胞形态上有明显差异，但表型和遗传比较结果表明新分离株与海洋卟啉杆菌密切相关。因此，我们将新分离株归入卟啉杆菌属，并为其提议新名称嗜温卟啉杆菌；嗜温卟啉杆菌的模式菌株是菌株OT3（= DSM 10595）。
