Acedo-Félix Evelia, Pérez-Martínez Gaspar
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado de Correos 73, 46100 - Burjassot, Valencia, Spain.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):67-75. doi: 10.1099/ijs.0.02325-0.
Many studies on Lactobacillus casei subsp. casei (L. casei) have been carried out using strain ATCC 393 (pLZ15-). Four strains of L. casei ATCC 393T and three of ATCC 393 (pLZ15-) were compared using phenotypic methods and many of the available genotyping techniques. These tests showed that strains of ATCC 393T obtained from independent public type-culture collections were significantly different from the plasmid-free (pLZ15-) strains of ATCC 393T. These findings were confirmed by sequencing the first 580 nt (domain I) of the 16S and 23S rDNAs of the strains. Complete sequencing of the 16S rDNA of one representative strain from each group revealed that strain ATCC 393T from culture collections was 99% similar to Lactobacillus zeae ATCC 15820T and that the strain so far considered as L. casei ATCC 393 (pLZ15-) was, in turn, 100% similar to L. casei ATCC 334 and Lactobacillus paracasei subsp. paracasei ATCC 4022. All data obtained in this work indicate that the ancestral strain of ATCC 393 (pLZ15-) might never have been the strain that is now available from culture collections.
许多关于干酪乳杆菌干酪亚种（L. casei）的研究都是使用菌株ATCC 393（pLZ15-）进行的。利用表型方法和许多现有的基因分型技术，对四株干酪乳杆菌ATCC 393T菌株和三株ATCC 393（pLZ15-）菌株进行了比较。这些测试表明，从独立的公共标准菌株保藏中心获得的ATCC 393T菌株与无质粒（pLZ15-）的ATCC 393T菌株存在显著差异。通过对这些菌株的16S和23S rDNA的前580 nt（结构域I）进行测序，证实了这些发现。对每组中的一个代表性菌株的16S rDNA进行全序列测定，结果显示，来自标准菌株保藏中心的ATCC 393T菌株与玉米乳杆菌ATCC 15820T的相似性为99%，而迄今为止被认为是干酪乳杆菌ATCC 393（pLZ15-）的菌株与干酪乳杆菌ATCC 334和副干酪乳杆菌副干酪亚种ATCC 4022的相似性为100%。这项工作中获得的所有数据表明，ATCC 393（pLZ15-）的原始菌株可能从来都不是现在从标准菌株保藏中心获得的那种菌株。
