Dicks L M, Du Plessis E M, Dellaglio F, Lauer E
Department of Microbiology, University of Stellenbosch, South Africa.
Int J Syst Bacteriol. 1996 Jan;46(1):337-40. doi: 10.1099/00207713-46-1-337.
The type strain of Lactobacillus casei subsp. casei (ATCC 393) exhibits low levels of DNA homology with other strains of L. casei subsp. casei (8 to 46%) and strains of Lactobacillus paracasei (30 to 50%), but exhibits a level of DNA similarity of 80% with Lactobacillus rhamnosus ATCC 15820, the original type strain of "Lactobacterium zeae" Kuznetsov 1959. Strains ATCC 393T (T = type strain) and ATCC 15820T are members of one protein profile cluster that is separate from the other Lactobacillus spp. The randomly amplified polymorphic DNA PCR profile of strain ATCC 393T is also different from the profiles obtained for the other species. L. casei ATCC 334T is genetically closely related to L. casei subsp. casei strains (71 to 97%) and L. paracasei strains (71 to 91%), is a member of the same protein profile cluster as these organisms, and shares several DNA amplicons with L. paracasei strains. On the basis of these results, we propose that L. casei subsp. casei ATCC 393T and L. rhamnosus ATCC 15820 should be reclassified as members of Lactobacillus zeae nom. rev. (type strain, ATCC 15820), that strain ATCC 334 should be designated the neotype strain of L. casei subsp. casei, and that the name L. paracasei should be rejected.
干酪乳杆菌干酪亚种的模式菌株（ATCC 393）与其他干酪乳杆菌干酪亚种菌株（8%至46%）以及副干酪乳杆菌菌株（30%至50%）的DNA同源性较低，但与鼠李糖乳杆菌ATCC 15820（“玉米乳杆菌”库兹涅佐夫1959年的原始模式菌株）的DNA相似性水平为80%。菌株ATCC 393T（T = 模式菌株）和ATCC 15820T属于一个蛋白质谱簇，该簇与其他乳酸杆菌属物种分开。菌株ATCC 393T的随机扩增多态性DNA PCR谱也与其他物种获得的谱不同。干酪乳杆菌ATCC 334T与干酪乳杆菌干酪亚种菌株（71%至97%）和副干酪乳杆菌菌株（71%至91%）在遗传上密切相关，是与这些生物体属于同一蛋白质谱簇的成员，并与副干酪乳杆菌菌株共享几个DNA扩增子。基于这些结果，我们建议将干酪乳杆菌干酪亚种ATCC 393T和鼠李糖乳杆菌ATCC 15820重新分类为玉米乳杆菌（Lactobacillus zeae）nom. rev.（模式菌株，ATCC 15820）的成员，将菌株ATCC 334指定为干酪乳杆菌干酪亚种的新模式菌株，并应摒弃副干酪乳杆菌这个名称。
