Losso Estela Maris, Nicolau José
Stomatology Department, Federal University of Paraná, Curitiba, PR, Brazil.
Braz Dent J. 2003;14(1):5-11. doi: 10.1590/s0103-64402003000100001. Epub 2003 Jul 31.
The objective of this study was to present a classification of the root development stage of female rat molar teeth and to evaluate the variation in the lactate dehydrogenase (LDH) activity and electrophoretic isoenzyme profile according to the stage of root development of the molar teeth. We also studied the LDH activity and isoenzymes of the pulp of incisor teeth. The stage of development of the rat first molar at the age of 15 days and that of the second molar at the age of 18 days was classified as the beginning of root formation. At the age of 15 days, the electrophoretic profile of the isoenzymes for the first molar showed a prevalence of LDH-1 followed by LDH-2. However, for the maxillary second molar there was a prevalence of LDH-4 followed by LDH-1, while for the mandibular second molar LDH-1 predominated followed by LDH-2 and LDH-4. From 18 days of age, the prevalence was always of LDH-1. The electrophoretic profile of LDH isoenzymes from the pulp of the incisor teeth at the ages studied (25 and 60 days) showed the following order of prevalence: LDH-1 > LDH-2 > LDH-3 > LDH-4 > LDH-5. These results suggest that there are variations in the prevalence of the various forms of LDH isoenzymes in the dental pulp of rats according to the developmental stage of the root.
本研究的目的是提出雌性大鼠磨牙牙根发育阶段的分类，并根据磨牙牙根发育阶段评估乳酸脱氢酶（LDH）活性和电泳同工酶谱的变化。我们还研究了切牙牙髓的LDH活性和同工酶。15日龄大鼠第一磨牙和18日龄大鼠第二磨牙的发育阶段被归类为牙根形成的开始。15日龄时，第一磨牙同工酶的电泳图谱显示LDH-1占优势，其次是LDH-2。然而，上颌第二磨牙以LDH-4占优势，其次是LDH-1，而下颌第二磨牙以LDH-1为主，其次是LDH-2和LDH-4。从18日龄开始，优势总是LDH-1。在所研究年龄（25天和60天）的切牙牙髓中，LDH同工酶的电泳图谱显示出以下优势顺序：LDH-1 > LDH-2 > LDH-3 > LDH-4 > LDH-5。这些结果表明，根据牙根的发育阶段，大鼠牙髓中各种形式的LDH同工酶的优势存在差异。
