Reddy Putta Mallikarjuna, Bruice Thomas C
Department of Chemistry and Biochemistry, University of California at Santa Barbara, CA 93106, USA.
Bioorg Med Chem Lett. 2003 Apr 7;13(7):1281-5. doi: 10.1016/s0960-894x(03)00119-7.
Positively charged DNG oligonucleotide mixed sequences containing A/T bases were prepared by solid-phase synthesis. Synthesis proceeds in 3'-->5' direction and involves coupling of 3'-Fmoc protected thiourea in the presence of HgCl(2)/TEA with the corresponding 5'-amine of the growing oligo chain. DNG binding characteristics with complementary DNA and with itself have been evaluated.
通过固相合成制备了含有A/T碱基的带正电荷的DNG寡核苷酸混合序列。合成沿3'→5'方向进行，包括在HgCl₂/TEA存在下，将3'-Fmoc保护的硫脲与生长的寡核苷酸链的相应5'-胺进行偶联。已经评估了DNG与互补DNA及其自身的结合特性。
