Cell-mediated immunity to cell wall antigens of Staphylococcus aureus in vitro. II. The production of chemotactic factor by guinea pig peritoneal exudate cells.

Peritoneal exudate cells from sensitized guinea pigs were incubated in culture with staphylococcal cell walls. Filtered supernatant fluids from the cultures were chemotactic for horse leukocytes. Maximum chemotactic activity was observed in supernatant fluids of cultures incubated 1.5 hours. Activity disappeared after 4.5 and 18 hours additional incubation.