Wang Cong-Yi, Shi Jing-Da, Yang Ping, Kumar Pradeep G, Li Quan-Zhen, Run Qing-Guo, Su Yun-Chao, Scott Hamish S, Kao Kuo-Jang, She Jin-Xiong
Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta, GA 30912, USA.
Gene. 2003 Mar 13;306:37-44. doi: 10.1016/s0378-1119(02)01210-6.
We have recently cloned four novel human genes that encode the ancient conserved domain proteins (ACDP). The full-length cDNA sequence of ACDP1 consists of 5898 bp and encodes a predicted protein of 951 amino acids (AA). The transcript for ACDP2 has 4058 bp of cDNA sequence, encoding a protein of 875 AA. ACDP3 contains 3113 bp of cDNA sequence and encodes a putative protein of 707 AA. ACDP4 contains 4765 bp of cDNA sequence and encodes a protein of 775 AA. The ACDP genes belong to a highly conserved new gene family. The conserved region showed 62.8% of nucleotide sequence identity, and 65.5% of AA identity with 92% of AA homologies among ACDP members. The conserved domain is also found in genes from evolutionarily divergent species from bacteria, yeast, Caenorhabditis elegans, and Drosophila melanogaster to mammals. All ACDP genes except ACDP1 have a ubiquitous expression pattern while ACDP1 expression is restricted to the brain and testis. Immunofluorescence staining of premeablized HeLa cells showed that ACDP proteins are predominantly localized in the nucleus. Sequence homology analyses revealed AA property and structural homologies between the ACD domain and cyclin molecules.
我们最近克隆了四个编码古老保守结构域蛋白（ACDP）的新型人类基因。ACDP1的全长cDNA序列由5898个碱基对组成，编码一个预测的含951个氨基酸（AA）的蛋白质。ACDP2的转录本具有4058个碱基对的cDNA序列，编码一个含875个AA的蛋白质。ACDP3包含3113个碱基对的cDNA序列，编码一个推测的含707个AA的蛋白质。ACDP4包含4765个碱基对的cDNA序列，编码一个含775个AA的蛋白质。ACDP基因属于一个高度保守的新基因家族。保守区域显示核苷酸序列同一性为62.8%，氨基酸同一性为65.5%，ACDP成员间的氨基酸同源性为92%。在从细菌、酵母、秀丽隐杆线虫、黑腹果蝇到哺乳动物等进化上不同的物种的基因中也发现了保守结构域。除ACDP1外，所有ACDP基因都有普遍的表达模式，而ACDP1的表达仅限于大脑和睾丸。对透化的HeLa细胞进行免疫荧光染色显示，ACDP蛋白主要定位于细胞核。序列同源性分析揭示了AC结构域与细胞周期蛋白分子之间的氨基酸特性和结构同源性。
