Oesser Steffen, Seifert Jürgen
Surgical Research of the Department of General Surgery and Thoracic Surgery of the University of Kiel, Michaelisstrasse 5, 24105, Kiel, Germany.
Cell Tissue Res. 2003 Mar;311(3):393-9. doi: 10.1007/s00441-003-0702-8. Epub 2003 Feb 25.
The functional integrity of articular cartilage is dependent on the maintenance of the extracellular matrix (ECM), a process which is controlled by chondrocytes. The regulation of ECM biosynthesis is complex and a variety of substances have been found to influence chondrocyte metabolism. In the present study we have investigated the effect of degraded collagen on the formation of type II collagen by mature bovine chondrocytes in a cell culture model. The culture medium was supplemented with collagen hydrolysate (CH) and biosynthesis of type II collagen by chondrocytes was compared to control cells treated with native type I and type II collagen and a collagen-free protein hydrolysate. The quantification of type II collagen by means of an ELISA technique was confirmed by immunocytochemical detection as well as by the incorporation of (14)C-proline in the ECM after a 48 h incubation. Chondrocytes in the control group were maintained in the basal medium for 11 days. The presence of extracellular CH led to a dose-dependent increase in type II collagen secretion. However, native collagens as well as a collagen-free hydrolysate of wheat proteins failed to stimulate the production of type II collagen in chondrocytes. These results clearly indicate a stimulatory effect of degraded collagen on the type II collagen biosynthesis of chondrocytes and suggest a possible feedback mechanism for the regulation of collagen turnover in cartilage tissue.
关节软骨的功能完整性依赖于细胞外基质（ECM）的维持，这一过程由软骨细胞控制。ECM生物合成的调节很复杂，已发现多种物质会影响软骨细胞代谢。在本研究中，我们在细胞培养模型中研究了降解胶原对成熟牛软骨细胞II型胶原形成的影响。向培养基中添加胶原水解物（CH），并将软骨细胞II型胶原的生物合成与用天然I型和II型胶原以及无胶原蛋白水解物处理的对照细胞进行比较。通过ELISA技术对II型胶原进行定量，经免疫细胞化学检测以及在48小时孵育后将（14）C-脯氨酸掺入ECM中得到证实。对照组的软骨细胞在基础培养基中培养11天。细胞外CH的存在导致II型胶原分泌呈剂量依赖性增加。然而，天然胶原以及小麦蛋白的无胶原水解物未能刺激软骨细胞中II型胶原的产生。这些结果清楚地表明降解胶原对软骨细胞II型胶原生物合成具有刺激作用，并提示了软骨组织中胶原周转调节的一种可能的反馈机制。
