Liang Qing-hua, He Jin-hua, Li Xia-ling
Institute of Combined Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Hunan Yi Ke Da Xue Xue Bao. 2002 Dec 28;27(6):491-4.
To observe the effect of bizhongxiao (BZX) decoction on the expression of VEGF in the synovial membrane of C II-induced arthritis(CIA) in rats and to explore the mechanism of BZX decoction in the treatment of rheumatoid arthritis RA.
Seventy-five SD rats were randomly divided into four groups. The rat experimental arthritis model was established by subcutaneous injection of II collogen. The expression of VEGF was detected with immunohistochemistry in different times.
The incidence of arthritis in the rats immunized with C II was approximately 88.57%. The arthritis index of the model group was rising, but that of the BZX decoction group and the methotrexate (MTX) group were decreasing on the 30th day. No expression of VEGF was found in the synovial membrane of the normal group. The expression of VEGF of the model group was notably higher than that of the BZX decoction group and the MTX group in different times (P < 0.01). The expression of VEGF of the model group rose step by step, but that of the BZX decoction group and the MTX group decreased and was significantly lower than that of the model group (P < 0.01). The expression of VEGF of the BZX decoction group was significantly lower than that of the MTX group(P < 0.05).
The expression of VEGF is relative to the synovial pannus formation in RA. BZX decoction and MTX could decrease the expression of VEGF, but the curative effect of BZX decoction is significantly better than that of MTX. BZX decoction could inhibit the formation of the synovial pannus or bone in RA by decreasing the expression of VEGF.
观察痹肿消（BZX）汤对Ⅱ型胶原诱导性关节炎（CIA）大鼠滑膜组织中血管内皮生长因子（VEGF）表达的影响，探讨BZX汤治疗类风湿关节炎（RA）的作用机制。
将75只SD大鼠随机分为4组。采用皮下注射Ⅱ型胶原的方法建立大鼠实验性关节炎模型。于不同时间用免疫组化法检测VEGF的表达。
Ⅱ型胶原免疫大鼠的关节炎发病率约为88.57%。模型组关节炎指数呈上升趋势，而BZX汤组和甲氨蝶呤（MTX）组在第30天时关节炎指数呈下降趋势。正常组滑膜组织中未发现VEGF表达。模型组VEGF表达在不同时间均显著高于BZX汤组和MTX组（P<0.01）。模型组VEGF表达呈逐步上升趋势，而BZX汤组和MTX组VEGF表达呈下降趋势，且显著低于模型组（P<0.01）。BZX汤组VEGF表达显著低于MTX组（P<0.05）。
VEGF表达与RA滑膜血管翳形成有关。BZX汤和MTX均可降低VEGF表达，但BZX汤疗效显著优于MTX。BZX汤可能通过降低VEGF表达抑制RA滑膜血管翳或骨的形成。
