Jun Tae-Youn, Pae Chi-Un, Kim Kwang-Soo, Han Hoon, Serretti Alessandro
Department of Psychiatry, College of Medicine, Catholic University of Korea, St Mary's Hospital, Seoul, Korea.
Psychiatry Clin Neurosci. 2003 Apr;57(2):153-9. doi: 10.1046/j.1440-1819.2003.01095.x.
The present study was aimed at examining the interleukin (IL)-10 gene promoter region polymorphic variants in patients with schizophrenia in the Korean population. Two hundred and thirty-three Korean patients diagnosed to have schizophrenia on the basis of Diagnostic and Statistical Manual of Mental Disorders (4th edn; DSM-IV) and 181 normal healthy controls participated in the present study. The DNA was extracted from whole blood using proteinase K and the IL-10 gene promoter region was amplified by polymerase chain reaction. Gene typing was performed by restriction fragment length polymorphism and single-strand conformation polymorphism. Distribution of the alleles and haplotypes in patients with schizophrenia was not significantly different from those of controls. The present study suggests that IL-10 gene promoter polymorphism is not associated with the development of schizophrenia in the Korean population.
本研究旨在检测韩国人群中精神分裂症患者白细胞介素（IL）-10基因启动子区域的多态性变异。根据《精神疾病诊断与统计手册》（第4版；DSM-IV）诊断为精神分裂症的233例韩国患者和181例正常健康对照参与了本研究。使用蛋白酶K从全血中提取DNA，并通过聚合酶链反应扩增IL-10基因启动子区域。通过限制性片段长度多态性和单链构象多态性进行基因分型。精神分裂症患者的等位基因和单倍型分布与对照组无显著差异。本研究表明，在韩国人群中，IL-10基因启动子多态性与精神分裂症的发生无关。
