Notch及其配体对32D细胞分化的影响

[Effects of Notch and its ligands on the differentiation of 32D cell].

作者信息

Ji Chunyan, Ma Daoxin, Zhao Jianqiang, Zhang Maohong

机构信息

Institute of Hematology, Shandong University Qilu Hospital, Jinan 250012, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2002 Dec;23(12):642-4.

PMID:12667348

Abstract

OBJECTIVE

To explore the mechanism of Notch signaling transduction system and its effects on hematopoietic system.

METHODS

Notch ligands transfected CHO cells were added into Notch1 and Notch2 transfected CHO cells, which were transiently transfected with reporter gene TP1. PGL-100 was used as substrate to test the interaction between Notch and Notch ligands. CHO, Jagged2-CHO and Delta 4-CHO cells were seeded in the petri dish containing G-CSF, and then Notch 1-32D cells were added in it to observe the differentiation of Notch1-32D cell after incubation and staining.

RESULTS

All of the five Notch ligands binding to Notch1 could induce TP1 activity, it increased significantly the Jagged2-CHO, Delta 4-CHO1-4 and Delta 4-CHO1-5 cells. For Notch2, the TP1 activity induced by the five ligands in these cells was much higher than that of CHO. At the presence of G-CSF, Notch1-32D could differentiate to mature granulocyte. Jagged2 could inhibit G-CSF induced Notch1-32D cell differentiation, but Delta 4 could not.

CONCLUSION

Jagged2 and Delta 4 are the ligands of Notch1. Jagged2 can inhibit G-CSF induced Notch1-32D cell differentiation, but Delta 4 can not.

摘要

目的

探讨Notch信号转导系统的机制及其对造血系统的影响。

方法

将Notch配体转染的CHO细胞加入到瞬时转染报告基因TP1的Notch1和Notch2转染的CHO细胞中。以PGL-100为底物检测Notch与Notch配体之间的相互作用。将CHO、Jagged2-CHO和Delta 4-CHO细胞接种于含G-CSF的培养皿中，然后加入Notch 1-32D细胞，孵育并染色后观察Notch1-32D细胞的分化情况。

结果

与Notch1结合的5种Notch配体均可诱导TP1活性，在Jagged2-CHO、Delta 4-CHO1-4和Delta 4-CHO1-5细胞中显著升高。对于Notch2，这5种配体在这些细胞中诱导的TP1活性远高于CHO细胞。在G-CSF存在的情况下，Notch1-32D可分化为成熟粒细胞。Jagged2可抑制G-CSF诱导的Notch1-32D细胞分化，但Delta 4不能。