Tanner G A, Sophasan S
Am J Physiol. 1976 Apr;230(4):1173-81. doi: 10.1152/ajplegacy.1976.230.4.1173.
Acute kidney failure was produced in the anesthetized rat by 1 h of complete renal artery occlusion. Kidney function was studied either immediately after release of the occlusion or 1 day later using clearance, micropuncture, histological, and nephron dissection techniques. Polyfructosan clearance was decreased to 5% of normal after temporary occlusion. Proximal tubular pressure (PTP) averaged 13-14 mmHg in normal kidneys, 39 mmHg immediately after release of unilateral occlusion, 19 mmHg 1 day after unilateral occlusion, and 25 mmHg 1 day after bilateral occlusion. The increased PTP reduces the glomerular filtration rate (GFR). Glomerular capillary pressure, estimated from the sum of the stop-flow and arterial plasma colloid osmotic pressures, was not decreased after temporary ischemia. Single-nephron GFR, measured without intratubular pressure control, was only slightly below normal 1 day after bilateral occlusion. Most distal tubules from ischemia-damaged kidneys contained hyaline casts. Tubular obstruction is a major factor in this model of acute kidney failure.
通过完全阻断肾动脉1小时，在麻醉大鼠中诱发急性肾衰竭。在解除阻断后立即或1天后，使用清除率、微穿刺、组织学和肾单位解剖技术研究肾功能。暂时阻断后，聚果糖清除率降至正常的5%。正常肾脏近端肾小管压力（PTP）平均为13 - 14 mmHg，单侧阻断解除后立即为39 mmHg，单侧阻断1天后为19 mmHg，双侧阻断1天后为25 mmHg。升高的PTP降低了肾小球滤过率（GFR）。根据停流和动脉血浆胶体渗透压之和估算的肾小球毛细血管压力，在暂时缺血后并未降低。在未控制肾小管内压力的情况下测量的单肾单位GFR，在双侧阻断1天后仅略低于正常水平。缺血损伤肾脏的大多数远端肾小管含有透明管型。肾小管阻塞是该急性肾衰竭模型中的一个主要因素。
