Dennis Philip C, Sleep Brent E, Fulthorpe Roberta R, Liss Steven N
Department of Civil Engineering, University of Toronto, 35 St. George St., Toronto, ON M5S 1A4, Canada.
Can J Microbiol. 2003 Jan;49(1):15-27. doi: 10.1139/w03-008.
An anaerobic microbial consortium able to biodegrade saturation levels of perchloroethylene (PCE) in a column containing a source zone of PCE was examined phylogenetically to determine microbial community structure and spatial variation in relation to the PCE source. The consortium was comprised of at least 34 members with 7 organisms sharing affiliations with known respiratory or cometabolic dechlorinators. Seven other organisms had their closest phylogenetic relative detected in other environments containing chlorinated compounds. Based on denaturing gradient gel electrophoresis, significant Bacteria were Dehalococcoides ethenogenes, Shewanella putrefaciens, and an Acetobacterium species. Spatial variations in community structure of the consortium relative to the PCE source zone were observed. A Pseudomonas species was predominant in a zone 30 cm from the PCE source. A Methanothrix species was predominant at points over 85 cm from the source zone. A Trichlorobacter species was detected where PCE concentrations were highest, up to 85 cm from the PCE source, whereas D. ethenogenes was ubiquitous to over 128 cm from the PCE source.
在一个含有全氯乙烯（PCE）源区的柱体中，对一个能够生物降解PCE饱和水平的厌氧微生物群落进行了系统发育研究，以确定微生物群落结构以及与PCE源相关的空间变化。该群落至少由34个成员组成，其中7种生物与已知的呼吸或共代谢脱氯菌有亲缘关系。另外7种生物在其他含有氯代化合物的环境中检测到了与其亲缘关系最近的生物。基于变性梯度凝胶电泳，重要的细菌有产乙烯脱卤球菌、腐败希瓦氏菌和一种醋杆菌属物种。观察到该群落相对于PCE源区的群落结构存在空间变化。在距离PCE源30厘米的区域，一种假单胞菌属物种占主导地位。在距离源区超过85厘米的点上，一种甲烷丝状菌属物种占主导地位。在PCE浓度最高的地方，即距离PCE源85厘米以内，检测到一种三氯杆菌属物种，而产乙烯脱卤球菌在距离PCE源超过128厘米的范围内普遍存在。
