通过竞争性酶联免疫吸附测定（c-ELISA）对病毒结构蛋白和非结构蛋白进行检测，以此确定马对马动脉炎病毒的血清学反应。
The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins.
作者信息
Wagner H M, Balasuriya U B R, James MacLachlan N
机构信息
Bernard and Gloria Salick Equine Viral Disease Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, 95616, Davis, CA, USA
出版信息
Comp Immunol Microbiol Infect Dis. 2003 Jul;26(4):251-60. doi: 10.1016/S0147-9571(02)00054-1.
In an effort to further characterize the humoral immune response of horses to equine arteritis virus (EAV), direct and competitive enzyme-linked immunosorbent assays (c-ELISAs) were developed using monoclonal and polyclonal anti-sera to structural (G(L), N and M) and non-structural (nsp1) viral proteins. A nsp1-specific monoclonal antibody was produced to facilitate development of a c-ELISA to this protein. Data obtained using the various c-ELISAs confirm that the M protein is a major target of the antibody response of horses to EAV. However, none of the c-ELISAs that were developed were as sensitive in detecting EAV-specific antibodies in horse sera as the existing serum neutralization test.
为了进一步描述马对马动脉炎病毒（EAV）的体液免疫反应，利用针对病毒结构蛋白（G(L)、N和M）和非结构蛋白（nsp1）的单克隆和多克隆抗血清，开发了直接和竞争性酶联免疫吸附测定（c-ELISA）。制备了一种nsp1特异性单克隆抗体，以促进针对该蛋白的c-ELISA的开发。使用各种c-ELISA获得的数据证实，M蛋白是马对EAV抗体反应的主要靶点。然而，所开发的c-ELISA在检测马血清中EAV特异性抗体方面，均不如现有的血清中和试验灵敏。
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