Postma D S, Pecorak J B, Prazma J, Logue S S, Fischer N D
Arch Otolaryngol. 1976 Mar;102(3):154-9. doi: 10.1001/archotol.1976.00780080076009.
A total of 26 albino guinea pigs were treated with 200 mg/kg/day of tobramycin sulfate. Animals were killed at various intervals of up to three weeks after seven days treatment. Outer hair cell (OHC) loss of the organ of Corti was evaluated by surface preparation techniques, and glycogen was assessed with PATCO- and PAS-stained sections. We have concluded that permanent damage of OHCs is most prevalent in areas that normally have the least amount and the smallest granule size of glycogen. With treatment, these susceptible areas are the least responsive in terms of an early increase in glycogen production. Furthermore, by 21 days after seven days of treatment, these same areas will reveal an almost total loss of glycogen at a time when damage is maximal.
总共26只白化豚鼠接受了每天200毫克/千克硫酸妥布霉素的治疗。在治疗7天后，动物在长达三周的不同时间间隔被处死。通过表面制备技术评估柯蒂氏器外毛细胞（OHC）的损失，并使用PATCO和PAS染色切片评估糖原。我们得出结论，OHC的永久性损伤在正常情况下糖原含量最少且颗粒尺寸最小的区域最为普遍。在治疗过程中，这些易感区域在糖原产生早期增加方面反应最小。此外，在治疗7天后的21天，当损伤最大时，这些相同区域将显示几乎完全失去糖原。
