Thalmann R, Paloheimo S, Thalmann I
Acta Otolaryngol. 1979 Mar-Apr;87(3-4):375-80. doi: 10.3109/00016487909126436.
Recent developments in radioimmunoassay technology have made possible measurements of cyclic nucleotides in individual specimens of the organ of Corti and its subdivisions. Steep longitudinal and transverse gradients of glycogen are known to exist in the organ of Corti of the guinea pig, with preferential accumulation in the outer hair cells of the apical turns. However, no significant longitudinal gradient of cyclic AMP was detectable in the organ of Corti, and the concentration of the compound was found to be nearly equal in the inner and outer hair cell layers. This is indirect evidence against the concept that cyclic AMP plays a role as "second messenger" in the control of glycogen metabolism of the organ of Corti. By contrast, the concentration of cyclic GMP was found to be consistently higher in the inner layer than in the outer layer of the organ of Corti, and to increase significantly in basal direction. This trend is remarkably similar to the distribution patterns of acetylcholinesterase, which may be considered as indirect evidence in favor of a possible role of cyclic GMP in the mediation of cholinergic effects.
放射免疫分析技术的最新进展使得对柯蒂氏器及其各亚部分的单个标本中的环核苷酸进行测量成为可能。已知豚鼠柯蒂氏器中存在陡峭的糖原纵向和横向梯度，且在顶端螺旋的外毛细胞中优先积累。然而，在柯蒂氏器中未检测到明显的环磷酸腺苷纵向梯度，并且发现该化合物在内外毛细胞层中的浓度几乎相等。这是反对环磷酸腺苷在柯蒂氏器糖原代谢控制中作为“第二信使”发挥作用这一概念的间接证据。相比之下，发现环磷酸鸟苷的浓度在柯蒂氏器的内层始终高于外层，并在基底方向上显著增加。这种趋势与乙酰胆碱酯酶的分布模式非常相似，这可被视为支持环磷酸鸟苷在胆碱能效应介导中可能发挥作用的间接证据。
