野生型和杂交苏云金芽孢杆菌δ-内毒素对小地老虎的活性。
Activity of wild-type and hybrid Bacillus thuringiensis delta-endotoxins against Agrotis ipsilon.
作者信息
de Maagd Ruud A, Weemen-Hendriks Mieke, Molthoff Jos W, Naimov Samir
机构信息
Plant Research International B.V., P.O. Box 16, 6700 AA, Wageningen, The Netherlands.
出版信息
Arch Microbiol. 2003 May;179(5):363-7. doi: 10.1007/s00203-003-0543-6. Epub 2003 Apr 2.
Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three active proteins were made by in vivo recombination and analyzed for activity against A. ipsilon. Analysis of hybrids between Cry1Aa and Cry1Fb indicated that domain I of Cry1Aa protein was involved in its higher activity.
对来自苏云金芽孢杆菌的12种Cry1和2种Cry9δ-内毒素进行了抗小地老虎（Agrotis ipsilon）活性测试。小地老虎对许多毒素不敏感，但有三种毒素具有显著活性。Cry9Ca毒性最强，其次是Cry1Aa和Cry1Fb。通过体内重组制备了这三种活性蛋白之间的杂种，并分析了其对小地老虎的活性。对Cry1Aa和Cry1Fb之间杂种的分析表明，Cry1Aa蛋白的结构域I涉及其更高的活性。
Zotero 插件