Yan Xiaorui, Lu Junjiao, Ren Meifeng, He Yin, Wang Yueqin, Wang Zhenying, He Kanglai
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China.
Insects. 2020 Mar 27;11(4):208. doi: 10.3390/insects11040208.
Black cutworm (BCW), (Hufnagel), is an occasional pest of maize that can cause considerable economic loss and injury to corn seedlings. This research mainly assessed the susceptibility of BCW neonates to 11 Bt toxins (Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, Cry1Ie, Cry1B, Cry2Aa, Vip3_ch1, Vip3_ch4, Vip3Ca2, Vip3Aa19) by exposing neonates to an artificial diet containing Bt toxins and evaluated the efficacy of three transgenic maize events (C008, C009, C010) expressing Vip3Aa19 toxin against BCW. The toxin-diet bioassay data indicated that Vip3Aa19 protein (LC = 0.43 μg/g) was the most active against BCW. Chimeric protein Vip3_ch1 (LC = 5.53 μg/g), Cry1F (LC = 83.62 μg/g) and Cry1Ac (LC = 184.77 μg/g) were less toxic. BCW was very tolerant to the other Bt toxins tested, with LC values more than 200 μg/g. Greenhouse studies were conducted with artificial infestations at the whorl stage by placing second-instar BCW larvae into whorl leaf and the fourth-instar larvae at the base of maize seedings. These results suggest that these transgenic maize events expressing Vip3Aa19 can provide effective control for BCW.
小地老虎（Black cutworm，BCW），（胡夫纳格尔），是玉米偶尔会遭遇的一种害虫，会给玉米幼苗造成相当大的经济损失和损害。本研究主要通过将小地老虎初孵幼虫暴露于含Bt毒素的人工饲料中，评估了小地老虎初孵幼虫对11种Bt毒素（Cry1Ab、Cry1Ac、Cry1Ah、Cry1F、Cry1Ie、Cry1B、Cry2Aa、Vip3_ch1、Vip3_ch4、Vip3Ca2、Vip3Aa19）的敏感性，并评价了三种表达Vip3Aa19毒素的转基因玉米事件（C008、C009、C010）对小地老虎的防治效果。毒素饲料生物测定数据表明，Vip3Aa19蛋白（LC = 0.43 μg/g）对小地老虎活性最强。嵌合蛋白Vip3_ch1（LC = 5.53 μg/g）、Cry1F（LC = 83.62 μg/g）和Cry1Ac（LC = 184.77 μg/g）毒性较小。小地老虎对所测试的其他Bt毒素具有很强的耐受性，LC值超过200 μg/g。通过在玉米喇叭口期人工接种，将二龄小地老虎幼虫放入喇叭口叶中，四龄幼虫放在玉米幼苗基部，进行了温室试验。这些结果表明，这些表达Vip3Aa19的转基因玉米事件可以有效防治小地老虎。
