癌胚抗原：高比活性亚组分的分离
Carcinoembryonic antigen: isolation of a sub-fraction with high specific activity.
作者信息
Rogers G T, Searle F, Bagshawe K D
出版信息
Br J Cancer. 1976 Apr;33(4):357-62. doi: 10.1038/bjc.1976.59.
Four sub-fractions of carcinoembryonic antigen have been obtained by chromatography of conventional CEA on Con A-Sepharose and their immunoreactive contents have been determined. Comparative studies have shown that a fraction eluted with 2% methyl glucoside (CEA 2B) had the highest activity, with a potency (60 u/mug) twice that of unfractionated CEA although appreciable activity was also found in the other fractions. The amino acid composition of CEA 2B is similar to that reported for conventional CEA but there is a lower content of neutral hexoses and a comparatively high content of N-acetylglucosamine. Experiments with a pool of sera from 11 patients with colonic cancer, which had been fractionated on Con A-Sepharose, have shown that nearly all the CEA activity was contained in a fraction eluted with 2% methyl glucoside. A convenient method of isolating CEA with high specific activity directly from perchloric acid extracts of tumour tissue is also described.
通过将传统癌胚抗原（CEA）在伴刀豆球蛋白A-琼脂糖凝胶上进行层析，已获得了CEA的四个亚组分，并测定了它们的免疫反应活性。比较研究表明，用2%甲基葡萄糖苷洗脱的一个组分（CEA 2B）具有最高活性，其效价（60u/μg）是未分级CEA的两倍，尽管在其他组分中也发现了相当可观的活性。CEA 2B的氨基酸组成与报道的传统CEA相似，但中性己糖含量较低，N-乙酰葡糖胺含量相对较高。对11例结肠癌患者的血清池在伴刀豆球蛋白A-琼脂糖凝胶上进行分级分离的实验表明，几乎所有的CEA活性都存在于用2%甲基葡萄糖苷洗脱的组分中。本文还描述了一种直接从肿瘤组织的高氯酸提取物中分离高比活性CEA的简便方法。
Zotero 插件