通过固相细胞术检测临床样本中的新型隐球菌单细胞
Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry.
作者信息
Bauters T G M, Swinne D, Stove V, Nelis H J
机构信息
Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1736-7. doi: 10.1128/JCM.41.4.1736-1737.2003.
Abstract
A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.
摘要
描述了一种基于固相细胞术的方法，用于检测和计数血清和脑脊液中的新型隐球菌单细胞。通过使用荧光活力标记和免疫荧光来检测活细胞和死细胞。这个30分钟的程序检测限为每毫升3至6个细胞。
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