用于检测脑脊液中新隐球菌的巢式聚合酶链反应的开发。
Development of a nested PCR for detection of Cryptococcus neoformans in cerebrospinal fluid.
作者信息
Rappelli P, Are R, Casu G, Fiori P L, Cappuccinelli P, Aceti A
机构信息
Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
出版信息
J Clin Microbiol. 1998 Nov;36(11):3438-40. doi: 10.1128/JCM.36.11.3438-3440.1998.
Abstract
We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conventional techniques and negative reactions for all 19 negative controls. Nested PCR is here compared with other diagnostic methods currently used in patients' follow-up exams during anticryptococcal therapy.
摘要
我们报告了一种基于巢式聚合酶链反应（nested-PCR）的检测脑脊液中新隐球菌的方法的开发情况。评估了该检测方法的特异性和灵敏度。然后将该技术应用于40份脑脊液样本。对于所有21份先前通过传统技术诊断为患有隐球菌性脑膜炎的患者的临床样本，我们均获得了阳性反应，而对于所有19份阴性对照样本均获得了阴性反应。本文将巢式PCR与目前在抗隐球菌治疗期间患者随访检查中使用的其他诊断方法进行了比较。
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本文引用的文献
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Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.肺隐球菌病患者中新型隐球菌基因的检测
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