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人类临床样本中荚膜组织胞浆菌荚膜变种的分子检测。

Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.

作者信息

Bracca Andrea, Tosello María Elena, Girardini Javier E, Amigot Susana L, Gomez Carlos, Serra Esteban

机构信息

Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina.

出版信息

J Clin Microbiol. 2003 Apr;41(4):1753-5. doi: 10.1128/JCM.41.4.1753-1755.2003.

DOI:10.1128/JCM.41.4.1753-1755.2003
PMID:12682178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC153865/
Abstract

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.

摘要

我们开发了一种用于诊断组织胞浆菌病的半巢式聚合酶链反应（PCR），该方法可扩增荚膜组织胞浆菌H抗原基因的一部分。此检测方法具有高度的敏感性和特异性，能够检测到对应少于10个酵母细胞的基因组物质，且不会与其他细菌或真菌病原体发生交叉反应。

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本文引用的文献

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Diagnosis of histoplasmosis by antigen detection based upon experience at the histoplasmosis reference laboratory.基于组织胞浆菌病参考实验室的经验，通过抗原检测诊断组织胞浆菌病。
Diagn Microbiol Infect Dis. 2002 May;43(1):29-37. doi: 10.1016/s0732-8893(02)00367-x.
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Comparison of an established antibody sandwich method with an inhibition method of Histoplasma capsulatum antigen detection.一种已确立的抗体夹心方法与荚膜组织胞浆菌抗原检测抑制法的比较。
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Genome size, complexity, and ploidy of the pathogenic fungus Histoplasma capsulatum.致病性真菌荚膜组织胞浆菌的基因组大小、复杂性和倍性。
J Bacteriol. 1998 Dec;180(24):6697-703. doi: 10.1128/JB.180.24.6697-6703.1998.
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Genomic diversity among Streptococcus agalactiae isolates detected by a degenerate oligonucleotide-primed amplification assay.通过简并寡核苷酸引物扩增分析法检测的无乳链球菌分离株之间的基因组多样性。
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Development of a novel antigen detection test for histoplasmosis.新型组织胞浆菌病抗原检测试验的开发。
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Cross-reactivity in Histoplasma capsulatum variety capsulatum antigen assays of urine samples from patients with endemic mycoses.地方性真菌病患者尿液样本在荚膜组织胞浆菌荚膜变种抗原检测中的交叉反应性。
Clin Infect Dis. 1997 Jun;24(6):1169-71. doi: 10.1086/513647.
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Pediatr Infect Dis J. 1994 Sep;13(9):801-5. doi: 10.1097/00006454-199409000-00010.
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Endemic mycoses in AIDS: a clinical review.艾滋病中的地方性真菌病：临床综述
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Disseminated histoplasmosis: clinical and pathologic correlations.播散性组织胞浆菌病：临床与病理相关性
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