人类临床样本中荚膜组织胞浆菌荚膜变种的分子检测。
Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.
作者信息
Bracca Andrea, Tosello María Elena, Girardini Javier E, Amigot Susana L, Gomez Carlos, Serra Esteban
机构信息
Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1753-5. doi: 10.1128/JCM.41.4.1753-1755.2003.
Abstract
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
摘要
我们开发了一种用于诊断组织胞浆菌病的半巢式聚合酶链反应（PCR），该方法可扩增荚膜组织胞浆菌H抗原基因的一部分。此检测方法具有高度的敏感性和特异性，能够检测到对应少于10个酵母细胞的基因组物质，且不会与其他细菌或真菌病原体发生交叉反应。
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