Ghaemi Mehran, Ahmadi Nasrollah, Sharifiyazdi Hassan, Ghane Mohsen, Golvajooei Mohamad Sadegh
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Vet Res Forum. 2022 Sep;13(3):455-459. doi: 10.30466/vrf.2021.539776.3237. Epub 2022 Sep 15.
is a dimorphic fungus that is traditionally classified in three varieties: var. , var. , and var. (HCF). Cytology, hematology, pathology, polymerase chain reaction (PCR), sequencing, and phylogenetic analyses were applied on samples collected from the blood and the eye of a horse with pustular lesions and ocular discharge. Physical examination and cytopathological tests showed infection. Additionally, the results of two PCR tests confirmed infection. The phylogenetic tree of the internal transcribed spacer sequence of Iranian showed homology with the HCF variety. For the first time, infection in the eye of a horse from Iran was detected and phylogenetically analyzed. This study revealed that could establish infection in Iranian animals in addition to people, and indicated the role of soil enriched with bird dropping in the preparation of a favorable environment for propagation. Further investigations are required to clarify the natural history and risk factors associated with histoplasmosis in Iran.
是一种双相真菌，传统上分为三个变种：变种、变种和变种（HCF）。对一匹患有脓疱性病变和眼部分泌物的马的血液和眼睛采集的样本进行了细胞学、血液学、病理学、聚合酶链反应（PCR）、测序和系统发育分析。体格检查和细胞病理学测试显示感染。此外，两次PCR测试结果证实了感染。伊朗的内部转录间隔区序列的系统发育树显示与HCF变种具有同源性。首次检测并对来自伊朗的一匹马眼睛中的感染进行了系统发育分析。这项研究表明，除了人类外，还可在伊朗动物中建立感染，并指出富含鸟粪的土壤在为传播创造有利环境中的作用。需要进一步调查以阐明伊朗组织胞浆菌病的自然史和相关危险因素。
