印度无偿献血者丙型肝炎病毒抗体检测中假阳性的出现情况。
Occurrence of false positives during testing for antibodies to hepatitis C virus among volunteer blood donors in India.
作者信息
Raghuraman Sukanya, Subramaniam Thenmozhi, Daniel Dolly, Sridharan Gopalan, Abraham Priya
机构信息
Department of Clinical Virology, Christian Medical College, Vellore 632 004, Tamil Nadu, India.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1788-90. doi: 10.1128/JCM.41.4.1788-1790.2003.
Abstract
The hepatitis C virus antibody statuses of only 11 (21.5%) of 51 initially reactive samples from volunteer blood donors could be confirmed by using additional screening and confirmatory assays; 23 (45%) were negative by all subsequent assays. Seventeen samples (33.3%) gave variable results in the different assays. The core and NS5 antigens were most immunogenic. An algorithm for serological screening of volunteer blood donors in blood banks of developing countries is suggested.
摘要
在51份来自无偿献血者的初始反应性样本中，只有11份（21.5%）的丙型肝炎病毒抗体状态能够通过额外的筛查和确证检测得以确认；23份（45%）在所有后续检测中均为阴性。17份样本（33.3%）在不同检测中结果不一。核心抗原和NS5抗原的免疫原性最强。本文提出了一种针对发展中国家血库中无偿献血者进行血清学筛查的方案。
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本文引用的文献
1
Blood transfusion practices in India: results of a national survey.
Indian J Gastroenterol. 2000 Apr-Jun;19(2):64-7.
2
Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.血液中免疫球蛋白G作为诊断性聚合酶链反应主要抑制剂的鉴定与表征
J Clin Microbiol. 2000 Jan;38(1):345-50. doi: 10.1128/JCM.38.1.345-350.2000.
3
4
Clinical significance of intrahepatic hepatitis C virus levels in patients with chronic HCV infection.慢性丙型肝炎病毒感染患者肝内丙型肝炎病毒水平的临床意义
Gut. 1998 Apr;42(4):570-5. doi: 10.1136/gut.42.4.570.
5
Lymphoproliferative responses to hepatitis C virus core, E1, E2, and NS3 in patients with chronic hepatitis C infection treated with interferon alfa.接受干扰素α治疗的慢性丙型肝炎感染患者对丙型肝炎病毒核心蛋白、E1、E2和NS3的淋巴细胞增殖反应。
Hepatology. 1996 Jan;23(1):8-16. doi: 10.1002/hep.510230102.
6
Diagnostic markers of viral hepatitis B and C.乙型和丙型病毒性肝炎的诊断标志物。
Gut. 1993;34(2 Suppl):S20-5. doi: 10.1136/gut.34.2_suppl.s20.
7
Use of several second generation serological assays to determine the true prevalence of hepatitis C virus infection in haemophiliacs treated with non-virus inactivated factor VIII and IX concentrates.使用多种第二代血清学检测方法来确定接受未进行病毒灭活的凝血因子VIII和IX浓缩物治疗的血友病患者中丙型肝炎病毒感染的真实患病率。
Br J Haematol. 1992 Apr;80(4):514-8. doi: 10.1111/j.1365-2141.1992.tb04566.x.
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J Clin Microbiol. 1992 Aug;30(8):2145-9. doi: 10.1128/jcm.30.8.2145-2149.1992.
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