Raziuddin S, Kawasaki T
Biochim Biophys Acta. 1976 Apr 22;431(1):116-26. doi: 10.1016/0005-2760(76)90265-4.
Lipopolysaccharides were isolated from the cell walls of Vibrio cholerae 569 B (Inaba) and El-tor (Inaba). Chemical analysis revealed the presence of glucose, fructose, mannose, heptose, rhamnose, ethanolamine, fatty acids and glucosamine. The lipopolysaccharides do not contain 2-keto-3-deoxyoctonate, the typical linking sugar of polysaccharide and lipid moieties of enterobacterial lipopolysaccharides. Galactose, a typical core polysaccharide component of many gram-negative bacteria was also absent from lipopolysaccharides of these organisms. By hydrolysis in 1% acetic acid, the lipopolysaccharides have been separated into a polysaccharide part (degraded polysaccharide) and a lipid part (lipid A). Components of degraded polysaccharide and lipid A moiety were identified and determined. The lipid A fractions contained fatty acids, phosphorus and glucosamine. All the neutral sugars detected in lipopolysaccharides were shown to be the constituents of its polysaccharide moiety. The fatty acid analysis of lipopolysaccharide and lipid A showed the presence of both hydroxy and non hydroxy acids. They were different from those of lipids extracted from cell walls before the extraction of lipopolysaccharides. 3-Hydroxylauric and 3-hydroxymyristic acids predominated in lipopolysaccharide and lipid A of Vibrio cholerae and El-tor (Inaba).
从霍乱弧菌569B（稻叶型）和埃尔托型（稻叶型）的细胞壁中分离出脂多糖。化学分析表明存在葡萄糖、果糖、甘露糖、庚糖、鼠李糖、乙醇胺、脂肪酸和氨基葡萄糖。这些脂多糖不含2-酮-3-脱氧辛酸，而2-酮-3-脱氧辛酸是肠道细菌脂多糖多糖和脂质部分的典型连接糖。许多革兰氏阴性菌典型的核心多糖成分半乳糖在这些生物体的脂多糖中也不存在。通过在1%乙酸中水解，脂多糖已被分离成多糖部分（降解多糖）和脂质部分（脂质A）。对降解多糖和脂质A部分的成分进行了鉴定和测定。脂质A部分含有脂肪酸、磷和氨基葡萄糖。脂多糖中检测到的所有中性糖均显示为其多糖部分的成分。脂多糖和脂质A的脂肪酸分析表明存在羟基酸和非羟基酸。它们与在提取脂多糖之前从细胞壁中提取的脂质的脂肪酸不同。3-羟基月桂酸和3-羟基肉豆蔻酸在霍乱弧菌和埃尔托型（稻叶型）的脂多糖和脂质A中占主导地位。
