Lohia A, Majumdar S, Chatterjee A N, Das J
J Bacteriol. 1985 Sep;163(3):1158-66. doi: 10.1128/jb.163.3.1158-1166.1985.
The rate and extent of lysis of Vibrio cholerae cells under nongrowing conditions were dependent on the osmolarity of the growth medium. Gross alterations in cellular morphology were observed when V. cholerae cells were grown in media of high and low osmolarity. The rate of lysis of V. cholerae cells under nongrowing conditions increased after treatment with chloramphenicol. Chloramphenicol-treated V. cholerae 569B cells showed formation of sphaeroplast-like bodies in medium of high osmolarity, but not in low osmolarity. Changes in the osmolarity of the growth medium also regulated the expression of the outer membrane proteins. This regulation was abolished if V. cholerae cells were grown in Pi-depleted medium. Analysis of the lytic behavior and composition of outer membrane proteins of an osmotically fragile mutant strain revealed a similar dependence on the osmolarity of the growth medium.
霍乱弧菌细胞在非生长条件下的裂解速率和程度取决于生长培养基的渗透压。当霍乱弧菌细胞在高渗和低渗培养基中生长时，可观察到细胞形态的总体变化。用氯霉素处理后，霍乱弧菌细胞在非生长条件下的裂解速率增加。经氯霉素处理的霍乱弧菌569B细胞在高渗培养基中形成了类似原生质球的物体，但在低渗培养基中则没有。生长培养基渗透压的变化也调节外膜蛋白的表达。如果霍乱弧菌细胞在缺磷培养基中生长，这种调节作用就会消失。对一株渗透敏感突变株的裂解行为和外膜蛋白组成的分析表明，其对生长培养基渗透压的依赖性相似。
