Iturbe-Ormaetxe Iñaki, Haralampidis Kosmas, Papadopoulou Kalliopi, Osbourn Anne E
Sainsbury Laboratory, John Innes Centre, Colney lane, Norwich NR4 7UH, U.K.
Plant Mol Biol. 2003 Mar;51(5):731-43. doi: 10.1023/a:1022519709298.
Cloning of OSCs required for triterpene synthesis from legume species that are amenable to molecular genetics will provide tools to address the importance of triterpenes and their derivatives during normal plant growth and development and also in interactions with symbionts and pathogens. Here we report the cloning and characterization of a total of three triterpene synthases from the legume species Medicago truncatula and Lotus japonicus. These include a beta-amyrin synthase from M. truncatula (MtAMY1) and a mixed function triterpene synthase from Lotus japonicus (LjAMY2). A partial cDNA predicted to encode a beta-amyrin synthase (LjAMY1) was also isolated from L. japonicus. The expression patterns of MtAMY1, LjAMY1 and LjAMY2 and of additional triterpene synthases previously characterised from M. truncatula and pea differ in different plant tissues and during nodulation, suggesting that these enzymes may have distinct roles in plant physiology and development.
从适合进行分子遗传学研究的豆科植物中克隆三萜合成所需的OSC，将为研究三萜及其衍生物在正常植物生长发育过程中以及与共生体和病原体相互作用中的重要性提供工具。在此，我们报告了从豆科植物蒺藜苜蓿和日本百脉根中总共克隆并鉴定出三种三萜合酶。其中包括来自蒺藜苜蓿的β-香树脂醇合酶（MtAMY1）和来自日本百脉根的多功能三萜合酶（LjAMY2）。还从日本百脉根中分离出一个预测编码β-香树脂醇合酶的部分cDNA（LjAMY1）。MtAMY1、LjAMY1和LjAMY2以及先前从蒺藜苜蓿和豌豆中鉴定出的其他三萜合酶在不同植物组织和结瘤过程中的表达模式不同，这表明这些酶在植物生理和发育中可能具有不同的作用。
