Luc G, Ferri res J, Evans A, Amouyel P, Arveiler D, Cambien F, Fruchart J-C, Ducimetière P
Département d'Athérosclérose, SERLIA-INSERM UR545, Institut Pasteur de Lille, 1, rue du Professeur-Calmette, 59019 Lille Cedex, France.
Rev Epidemiol Sante Publique. 2003 Feb;51(1 Pt 2):159-66.
Cardiovascular disease is the leading cause of mortality in westernized countries. Learning more about the cause of coronary heart disease (CHD) is an essential step in the search for effective CHD prevention, both at the individual and population levels. Prospective cohort studies are particularly well suited to the study of risk markers. However, the high cost of mounting such studies, along with the newer hypotheses generated during the period of follow-up necessitates the use of plasma and serum banks for analyses of many biological parameters.
The prospective, cohort PRIME Study has recruited 10,592 men, aged 50-59 years in France and Northern Ireland, to establish new risk markers for CHD. A plasma serum bank was established comprising 240,000 samples, either in straws or tubes, which have been stored in liquid nitrogen for over 5 years. The use of straws was required to store the largest number of aliquots in the smallest possible space. Storage validation was carried out for a number of key parameters. The validity of freezing of plasma in straws was established for a number of key measurements under investigation. Simultaneously, a DNA bank was set up to facilitate genetic analyses. In contrast to the DNA bank, which enables the performance of a very large number of analyses on a small amount of material, the plasma/serum bank has to be managed very frugally, requiring laboratories to use the smallest volume possible in each analysis.
Problems and difficulties solved during building and use of biological banks are presented. The initial results obtained using this plasma bank have demonstrated its validity.
心血管疾病是西方国家的主要死因。更多地了解冠心病（CHD）的病因是在个体和人群层面寻求有效预防冠心病的关键一步。前瞻性队列研究特别适合于风险标志物的研究。然而，开展此类研究成本高昂，且随访期间会产生新的假设，因此有必要利用血浆和血清库来分析多种生物学参数。
前瞻性队列PRIME研究招募了法国和北爱尔兰的10592名年龄在50至59岁之间的男性，以确定冠心病的新风险标志物。建立了一个血浆血清库，包含240000份样本，样本以麦管或试管形式保存，已在液氮中储存超过5年。使用麦管以便在尽可能小的空间内储存最多的分装样本。对一些关键参数进行了储存验证。确定了在研究中的一些关键测量指标下，血浆在麦管中冷冻的有效性。同时，建立了一个DNA库以促进基因分析。与能够对少量材料进行大量分析的DNA库不同，血浆/血清库必须非常节俭地管理，要求实验室在每次分析中使用尽可能小的体积。
介绍了生物样本库建立和使用过程中解决的问题和困难。使用该血浆库获得的初步结果证明了其有效性。
